Method for identifying wuzhishan miniature pig inbred line by using 145 snps

ABSTRACT

The present invention discloses a method for identifying Wuzhishan miniature pig inbred line by using 145 SNPs. The method for auxiliarily identifying whether a pig to be tested is Wuzhishan miniature pig inbred line, provided by the present invention, comprises the following steps: testing genotypes based on 145 SNP sites of the pig to be tested; if all standards of (1) to (145) are satisfied, the pig to be tested is a candidate for Wuzhishan miniature pig inbred line; if all the standards of the above (1) to (145) are not satisfied, the pig to be tested is a candidate for non-Wuzhishan miniature pig inbred line.

TECHNICAL FIELD

The present invention relates to a method for identifying Wuzhishan miniature pig inbred line by using 145 SNPs.

BACKGROUND

During the period of 1989-2003, under the support from seven related programs including the 7^(th), 8^(th) and 9^(th) Five-Year key industrial R & D Programs of China's Ministry of Agriculture, and “Chinese National Programs for High Technology Research and Development Program (863 Program)” of China's Ministry of Science and Technology, key and major projects of National Natural Science Foundation of China etc., the research working on “the breeding, germplasm characteristics, as well as development and utilization of Wuzhishan inbred line” had made many breakthroughs, including but not limited to, taking a boar and a sow from Wuzhishan pigs (also referred as Wuzhishan miniature pigs) as line ancestors, continuously employing “offspring-parent”, “full sibs” mating, and other comprehensive measures, gradually overcoming the adverse effects that inbreeding resulted in very low breeding survival rate, which was less than 20% at that time, establishing F₁₇ inbred line population and genealogy, monitoring the research process of inbred line breeding of inbred line varieties F₇-F₁₆ by using many advanced technical means, such as molecularly genetic means, DNA fingerprinting similarity factor, microsatellite etc., preliminarily revealing genetic regularity and characteristics of Wuzhishan miniature pig inbred line, and demonstrating the facticity and scientificity of the breeding process of this inbred line. Moreover, many studies on exploitation and utilization of Wuzhishan miniature pig inbred line had been completed, showing the incomparable superiority of this inbred line in serving as an ideal animal model for human disease, and consequently achieving a certain economic benefits and major social benefits. Two achievements of the researches—“Researches on germplasm characteristics as well as exploitation and utilization of Wuzhishan miniature pigs” (Achievements Appraisal of Academy of Agricultural Sciences [95], No. 075) and “Wuzhishan miniature pig inbred line breeding for experiments and molecule genetic basic research” passed the Science and Technology Achievement Appraisals hosted by China's Ministry of Agriculture in 1995 and 2005 respectively, and won the Third Prize of China's Ministry of Agriculture Science and Technology Progress Award in 1999.

Preliminary research achievements of miniature pig inbred breeding had great influences at home and abroad. For example, industrial specialists from the United States, Italy, Japan, West Germany, Korea, and other countries had proposed for introduction and cultivation, and or cooperative study. An institute of the United States proposed to purchase 50 boars with 5 million USD in 1996; Korea proposed to cooperatively set up factories in China by funding 5 million RMB in 2000 after its introduction and cultivation proposal was rejected. The main reason behind the influences is that the inbred line animals, as special genetic resources for animals, can rapidly obtain scientific data for life science researches with the same extent of sensitivity and accuracy as from analytical reagents in chemistry and precise instruments in physics. With their very high use value and research significance, more than 450 inbred lines of mice, rats, and small mammals etc, have been bred in the world at present, and widely used as animal models for studying difficult and complicated human diseases, solving difficult problems in bioscience, medical science, and pharmaceutics, and other research fields. Due to the various differences between pigs and those small animals in interspecific physiology, the breeding of inbred line pigs has been very difficult. It was verified by novelty searching (2013. 11) that there was no successful report in the world. Moreover, compared with rodents, pigs possesses greater similarity with human, and will play irreplaceable, unique roles in the researches for studying difficult and complicated human diseases and solving difficult problems in bioscience, medical science, and pharmaceutics. Inbred line pigs are rare, valuable genetic resources of large animals in the world. Previously, in 1960s, many departments of the United States and Europe carried out the breeding research of inbred line pigs, but failed with the highest inbreeding coefficient no more than 0.75. After a 15-year breeding research, China has obtained F₁₇ with the special Chinese miniature pig resource, and the inbreeding coefficient is up to 0.965, and more encouragingly the high incidence stage of piglet death due to inbred breeding has been overcome. Therefore, the successful breeding of inbred line pigs will be likely achieved in China firstly. After a further work in current years, Wuzhishan miniature pig inbred lines F₁₇ to F₂₂ have been obtained.

Because there was no successful breeding report for inbred line pigs in the world yet, and either the standard of establishment or identification method of inbred line pigs does not exist, either, we strictly referred to and followed the breeding method of inbred line mouse or rat, that was, inbreeding more than 20 generations from two pigs in same ancestor and establishing complete genealogy, testing the genetic stability thereof, establishing identification method, establishing line standard, specially on interspecific differences from current Hainan Wuzhishan pigs. Only in this way, scientific and credible evidence can be provided to the industry and the world, and such novel innovation project of genetic resource can be accomplished, thereby to benefit human beings.

Once the miniature pig inbred line is successfully bred, China will possess the proprietary intellectual property rights and the international leading level of innovative genetic germplasm resource of pig, which will fill up research blank within this research field in the world, and enrich theory and practice of inbred line breeding for large animals. Meanwhile, as an ideal “animal models” for human beings, inbred line pig is an important foundation and innovation basis for the progress of biotechnological research, which will significantly promote collaborative development and innovation in bioscience, human medical science, and pharmacy, etc. in China, and play irreplaceable position for studying difficult and complicated human diseases, extending lives, so as to benefit human beings. Therefore, it possesses important practical and theoretical significance.

THE CONTENT OF THE INVENTION

The purpose of the present invention is to provide a method for identifying Wuzhishan miniature pig inbred line by using 145 SNPs.

The present invention provides a method for auxiliarily identifying whether the pig to be tested is inbred line of Wuzhishan miniature pig, comprising the following steps:

Testing genotypes based on 145 SNP sites of the pig to be tested; If all the standards of the following (1) to (145) are satisfied, the pig to be tested is a candidate for Wuzhishan miniature pig inbred line:

-   -   (1) M1GA0025062 the genotype of SNP site is GG;     -   (2) DRGA0017627 the genotype of SNP site is GG;     -   (3) H3GA0056137 the genotype of SNP site is CC;     -   (4) MARC0039661 the genotype of SNP site is AA;     -   (5) ASGA0085025 the genotype of SNP site is TT;     -   (6) ASGA0098570 the genotype of SNP site is GG;     -   (7) MARC0063358 the genotype of SNP site is AA;     -   (8) ASGA0085437 the genotype of SNP site is GG;     -   (9) MARC0096709 the genotype of SNP site is CC;     -   (10) H3GA0052387 the genotype of SNP site is GG;     -   (11) ASGA0000264 the genotype of SNP site is TT;     -   (12) ALGA0000195 the genotype of SNP site is TT;     -   (13) H3GA0001444 the genotype of SNP site is AA;     -   (14) ASGA0002293 the genotype of SNP site is CC;     -   (15) INRA0001752 the genotype of SNP site is GG;     -   (16) H3GA0001445 the genotype of SNP site is GG;     -   (17) ALGA0002595 the genotype of SNP site is TT;     -   (18) ALGA0002600 the genotype of SNP site is GG;     -   (19) DRGA0000563 the genotype of SNP site is CC;     -   (20) DRGA0000565 the genotype of SNP site is CC;     -   (21) INRA0001761 the genotype of SNP site is AA;     -   (22) MARC0104045 the genotype of SNP site is CC;     -   (23) ALGA0002601 the genotype of SNP site is CC;     -   (24) DRGA0000569 the genotype of SNP site is AA;     -   (25) ALGA0002608 the genotype of SNP site is TT;     -   (26) DRGA0000568 the genotype of SNP site is CC;     -   (27) ALGA0002604 the genotype of SNP site is GG;     -   (28) MARC0048118 the genotype of SNP site is TT;     -   (29) ASGA0004229 the genotype of SNP site is CC;     -   (30) MARC0007088 the genotype of SNP site is CC;     -   (31) MARC0000061 the genotype of SNP site is GG;     -   (32) MARC0094747 the genotype of SNP site is AA;     -   (33) MARC0007969 the genotype of SNP site is AA;     -   (34) ASGA0106092 the genotype of SNP site is CC;     -   (35) ALGA0119806 the genotype of SNP site is GG;     -   (36) INRA0003745 the genotype of SNP site is AA;     -   (37) INRA0003746 the genotype of SNP site is TT;     -   (38) ALGA0005490 the genotype of SNP site is CC;     -   (39) H3GA0002538 the genotype of SNP site is CC;     -   (40) INRA0003749 the genotype of SNP site is CC;     -   (41) H3GA0002539 the genotype of SNP site is GG;     -   (42) M1GA0001099 the genotype of SNP site is CC;     -   (43) MARC0111831 the genotype of SNP site is AA;     -   (44) ASGA0005566 the genotype of SNP site is CC;     -   (45) ALGA0007578 the genotype of SNP site is CC;     -   (46) ASGA0005568 the genotype of SNP site is CC;     -   (47) INRA0005765 the genotype of SNP site is AA;     -   (48) MARC0095347 the genotype of SNP site is CC;     -   (49) MARC0039015 the genotype of SNP site is CC;     -   (50) DRGA0001892 the genotype of SNP site is GG;     -   (51) ALGA0007580 the genotype of SNP site is GG;     -   (52) ALGA0007583 the genotype of SNP site is TT;     -   (53) MARC0095915 the genotype of SNP site is CC;     -   (54) ASGA0005571 the genotype of SNP site is AA;     -   (55) MARC0014134 the genotype of SNP site is AA;     -   (56) MARC0024233 the genotype of SNP site is AA;     -   (57) H3GA0003548 the genotype of SNP site is GG;     -   (58) ALGA0007591 the genotype of SNP site is CC;     -   (59) MARC0000195 the genotype of SNP site is CC;     -   (60) ALGA0038697 the genotype of SNP site is CC;     -   (61) H3GA0020000 the genotype of SNP site is CC;     -   (62) ALGA0038703 the genotype of SNP site is CC;     -   (63) H3GA0020002 the genotype of SNP site is GG;     -   (64) DRGA0007138 the genotype of SNP site is CC;     -   (65) ASGA0031284 the genotype of SNP site is CC;     -   (66) ASGA0031285 the genotype of SNP site is AA;     -   (67) MARC0025042 the genotype of SNP site is GG;     -   (68) H3GA0020006 the genotype of SNP site is TT;     -   (69) ALGA0038729 the genotype of SNP site is CC;     -   (70) ALGA0038731 the genotype of SNP site is GG;     -   (71) ASGA0031321 the genotype of SNP site is GG;     -   (72) ASGA0031322 the genotype of SNP site is GG;     -   (73) ALGA0038747 the genotype of SNP site is AA;     -   (74) ASGA0033095 the genotype of SNP site is TT;     -   (75) ALGA0040854 the genotype of SNP site is GG;     -   (76) ALGA0040856 the genotype of SNP site is AA;     -   (77) ALGA0040857 the genotype of SNP site is GG;     -   (78) ASGA0033096 the genotype of SNP site is GG;     -   (79) INRA0025180 the genotype of SNP site is AA;     -   (80) ALGA0040859 the genotype of SNP site is CC;     -   (81) H3GA0021216 the genotype of SNP site is CC;     -   (82) ASGA0033098 the genotype of SNP site is CC;     -   (83) H3GA0021221 the genotype of SNP site is CC;     -   (84) MARC0014540 the genotype of SNP site is CC;     -   (85) ASGA0033103 the genotype of SNP site is CC;     -   (86) DIAS0000557 the genotype of SNP site is AA;     -   (87) ASGA0033116 the genotype of SNP site is AA;     -   (88) ASGA0036835 the genotype of SNP site is CC;     -   (89) MARC0031932 the genotype of SNP site is CC;     -   (90) ASGA0036838 the genotype of SNP site is CC;     -   (91) H3GA0023523 the genotype of SNP site is GG;     -   (92) ALGA0045460 the genotype of SNP site is TT;     -   (93) MARC0005927 the genotype of SNP site is GG;     -   (94) MARC0005928 the genotype of SNP site is AA;     -   (95) ASGA0036842 the genotype of SNP site is CC;     -   (96) ASGA0036846 the genotype of SNP site is CC;     -   (97) MARC0098637 the genotype of SNP site is TT;     -   (98) M1GA0011035 the genotype of SNP site is TT;     -   (99) DRGA0008230 the genotype of SNP site is GG;     -   (100) ASGA0036855 the genotype of SNP site is CC;     -   (101) ALGA0097277 the genotype of SNP site is GG;     -   (102) MARC0080197 the genotype of SNP site is CC;     -   (103) H3GA0050489 the genotype of SNP site is TT;     -   (104) ALGA0097281 the genotype of SNP site is TT;     -   (105) H3GA0050490 the genotype of SNP site is CC;     -   (106) ALGA0097282 the genotype of SNP site is TT;     -   (107) ASGA0079089 the genotype of SNP site is CC;     -   (108) INRA0055354 the genotype of SNP site is GG;     -   (109) ASGA0079091 the genotype of SNP site is TT;     -   (110) ASGA0079090 the genotype of SNP site is CC;     -   (111) H3GA0050491 the genotype of SNP site is AA;     -   (112) MARC0056017 the genotype of SNP site is TT;     -   (113) MARC0055759 the genotype of SNP site is AA;     -   (114) H3GA0050495 the genotype of SNP site is GG;     -   (115) ALGA0097291 the genotype of SNP site is GG;     -   (116) ALGA0097290 the genotype of SNP site is GG;     -   (117) CASI0006683 the genotype of SNP site is GG;     -   (118) ASGA0079098 the genotype of SNP site is CC;     -   (119) ALGA0097297 the genotype of SNP site is GG;     -   (120) H3GA0054426 the genotype of SNP site is TT;     -   (121) ALGA0098112 the genotype of SNP site is GG;     -   (122) MARC0089391 the genotype of SNP site is GG;     -   (123) ASGA0089892 the genotype of SNP site is AA;     -   (124) ASGA0097792 the genotype of SNP site is CC;     -   (125) MARC0046857 the genotype of SNP site is GG;     -   (126) MARC0077194 the genotype of SNP site is AA;     -   (127) ALGA0108769 the genotype of SNP site is CC;     -   (128) H3GA0050799 the genotype of SNP site is CC;     -   (129) ALGA0098120 the genotype of SNP site is CC;     -   (130) ALGA0098123 the genotype of SNP site is GG;     -   (131) ALGA0098128 the genotype of SNP site is GG;     -   (132) ASGA0079719 the genotype of SNP site is CC;     -   (133) ASGA0079728 the genotype of SNP site is GG;     -   (134) ASGA0080429 the genotype of SNP site is CC;     -   (135) MARC0052755 the genotype of SNP site is GG;     -   (136) ALGA0098918 the genotype of SNP site is GG;     -   (137) ASGA0080432 the genotype of SNP site is GG;     -   (138) ALGA0098922 the genotype of SNP site is CC;     -   (139) INRA0056206 the genotype of SNP site is GG;     -   (140) INRA0056207 the genotype of SNP site is CC;     -   (141) ASGA0080435 the genotype of SNP site is CC;     -   (142) MARC0068495 the genotype of SNP site is GG;     -   (143) ASGA0080436 the genotype of SNP site is CC;     -   (144) MARC0003370 the genotype of SNP site is CC;     -   (145) ASGA0085659 the genotype of SNP site is CC;

If any standard of the above is not satisfied, the pig to be tested is a candidate for non-Wuzhishan miniature pig inbred line.

The pig to be tested can be the Wuzhishan miniature pig inbred line or Hainan Wuzhishan pig.

The Wuzhishan miniature pig inbred line can belong to any one of F₂₀ to F₂₂ generations.

The Wuzhishan miniature pig inbred line can belong to F₂₀ generation or generations later than F₂₀ generation.

In this method, specific chips can be employed to test the genotype based on 145 SNP sites of the pig to be tested; the specific chips are nucleic acid chips in which single-stranded DNA molecules shown as Sequences 1-145 in the sequence listing are immobilized at different points, respectively.

The present invention is also used to protect a nucleic acid chip in which single-stranded DNA molecules shown as Sequences 1-145 in the sequence listing are immobilized at different points, respectively.

The present invention is also used to protect the use of the nucleic acid chip, which is the following (a) or (b): (a) auxiliarily identifying whether the pig to be tested is Wuzhishan miniature pig inbred line; or (b) auxiliarily identifying whether the pig population to be tested is Wuzhishan miniature pig inbred line population.

The pig to be tested can be the Wuzhishan miniature pig inbred line or Hainan Wuzhishan pig.

The Wuzhishan miniature pig inbred line can belong to any one of F₂₀ to F₂₂ generations.

The Wuzhishan miniature pig inbred line can belong to F₂₀ generation or generations later than F₂₀ generation.

The present invention also provides a method for auxiliarily identifying whether the pig population to be tested is Wuzhishan miniature pig inbred line population, comprising the following steps:

Randomly sampling from the pig population to be tested to obtain samples to be tested, then testing the genotypes based on 145 SNP sites of each of samples to be tested, and if all the samples to be tested satisfy all the standards of the following (1) to (145), the pig population to be tested is a candidate for Wuzhishan miniature pig inbred line population:

-   -   (1) M1GA0025062 the genotype of SNP site is GG;     -   (2) DRGA0017627 the genotype of SNP site is GG;     -   (3) H3GA0056137 the genotype of SNP site is CC;     -   (4) MARC0039661 the genotype of SNP site is AA;     -   (5) ASGA0085025 the genotype of SNP site is TT;     -   (6) ASGA0098570 the genotype of SNP site is GG;     -   (7) MARC0063358 the genotype of SNP site is AA;     -   (8) ASGA0085437 the genotype of SNP site is GG;     -   (9) MARC0096709 the genotype of SNP site is CC;     -   (10) H3GA0052387 the genotype of SNP site is GG;     -   (11) ASGA0000264 the genotype of SNP site is TT;     -   (12) ALGA0000195 the genotype of SNP site is TT;     -   (13) H3GA0001444 the genotype of SNP site is AA;     -   (14) ASGA0002293 the genotype of SNP site is CC;     -   (15) INRA0001752 the genotype of SNP site is GG;     -   (16) H3GA0001445 the genotype of SNP site is GG;     -   (17) ALGA0002595 the genotype of SNP site is TT;     -   (18) ALGA0002600 the genotype of SNP site is GG;     -   (19) DRGA0000563 the genotype of SNP site is CC;     -   (20) DRGA0000565 the genotype of SNP site is CC;     -   (21) INRA0001761 the genotype of SNP site is AA;     -   (22) MARC0104045 the genotype of SNP site is CC;     -   (23) ALGA0002601 the genotype of SNP site is CC;     -   (24) DRGA0000569 the genotype of SNP site is AA;     -   (25) ALGA0002608 the genotype of SNP site is TT;     -   (26) DRGA0000568 the genotype of SNP site is CC;     -   (27) ALGA0002604 the genotype of SNP site is GG;     -   (28) MARC0048118 the genotype of SNP site is TT;     -   (29) ASGA0004229 the genotype of SNP site is CC;     -   (30) MARC0007088 the genotype of SNP site is CC;     -   (31) MARC0000061 the genotype of SNP site is GG;     -   (32) MARC0094747 the genotype of SNP site is AA;     -   (33) MARC0007969 the genotype of SNP site is AA;     -   (34) ASGA0106092 the genotype of SNP site is CC;     -   (35) ALGA0119806 the genotype of SNP site is GG;     -   (36) INRA0003745 the genotype of SNP site is AA;     -   (37) INRA0003746 the genotype of SNP site is TT;     -   (38) ALGA0005490 the genotype of SNP site is CC;     -   (39) H3GA0002538 the genotype of SNP site is CC;     -   (40) INRA0003749 the genotype of SNP site is CC;     -   (41) H3GA0002539 the genotype of SNP site is GG;     -   (42) M1GA0001099 the genotype of SNP site is CC;     -   (43) MARC0111831 the genotype of SNP site is AA;     -   (44) ASGA0005566 the genotype of SNP site is CC;     -   (45) ALGA0007578 the genotype of SNP site is CC;     -   (46) ASGA0005568 the genotype of SNP site is CC;     -   (47) INRA0005765 the genotype of SNP site is AA;     -   (48) MARC0095347 the genotype of SNP site is CC;     -   (49) MARC0039015 the genotype of SNP site is CC;     -   (50) DRGA0001892 the genotype of SNP site is GG;     -   (51) ALGA0007580 the genotype of SNP site is GG;     -   (52) ALGA0007583 the genotype of SNP site is TT;     -   (53) MARC0095915 the genotype of SNP site is CC;     -   (54) ASGA0005571 the genotype of SNP site is AA;     -   (55) MARC0014134 the genotype of SNP site is AA;     -   (56) MARC0024233 the genotype of SNP site is AA;     -   (57) H3GA0003548 the genotype of SNP site is GG;     -   (58) ALGA0007591 the genotype of SNP site is CC;     -   (59) MARC0000195 the genotype of SNP site is CC;     -   (60) ALGA0038697 the genotype of SNP site is CC;     -   (61) H3GA0020000 the genotype of SNP site is CC;     -   (62) ALGA0038703 the genotype of SNP site is CC;     -   (63) H3GA0020002 the genotype of SNP site is GG;     -   (64) DRGA0007138 the genotype of SNP site is CC;     -   (65) ASGA0031284 the genotype of SNP site is CC;     -   (66) ASGA0031285 the genotype of SNP site is AA;     -   (67) MARC0025042 the genotype of SNP site is GG;     -   (68) H3GA0020006 the genotype of SNP site is TT;     -   (69) ALGA0038729 the genotype of SNP site is CC;     -   (70) ALGA0038731 the genotype of SNP site is GG;     -   (71) ASGA0031321 the genotype of SNP site is GG;     -   (72) ASGA0031322 the genotype of SNP site is GG;     -   (73) ALGA0038747 the genotype of SNP site is AA;     -   (74) ASGA0033095 the genotype of SNP site is TT;     -   (75) ALGA0040854 the genotype of SNP site is GG;     -   (76) ALGA0040856 the genotype of SNP site is AA;     -   (77) ALGA0040857 the genotype of SNP site is GG;     -   (78) ASGA0033096 the genotype of SNP site is GG;     -   (79) INRA0025180 the genotype of SNP site is AA;     -   (80) ALGA0040859 the genotype of SNP site is CC;     -   (81) H3GA0021216 the genotype of SNP site is CC;     -   (82) ASGA0033098 the genotype of SNP site is CC;     -   (83) H3GA0021221 the genotype of SNP site is CC;     -   (84) MARC0014540 the genotype of SNP site is CC;     -   (85) ASGA0033103 the genotype of SNP site is CC;     -   (86) DIAS0000557 the genotype of SNP site is AA;     -   (87) ASGA0033116 the genotype of SNP site is AA;     -   (88) ASGA0036835 the genotype of SNP site is CC;     -   (89) MARC0031932 the genotype of SNP site is CC;     -   (90) ASGA0036838 the genotype of SNP site is CC;     -   (91) H3GA0023523 the genotype of SNP site is GG;     -   (92) ALGA0045460 the genotype of SNP site is TT;     -   (93) MARC0005927 the genotype of SNP site is GG;     -   (94) MARC0005928 the genotype of SNP site is AA;     -   (95) ASGA0036842 the genotype of SNP site is CC;     -   (96) ASGA0036846 the genotype of SNP site is CC;     -   (97) MARC0098637 the genotype of SNP site is TT;     -   (98) M1GA0011035 the genotype of SNP site is TT;     -   (99) DRGA0008230 the genotype of SNP site is GG;     -   (100) ASGA0036855 the genotype of SNP site is CC;     -   (101) ALGA0097277 the genotype of SNP site is GG;     -   (102) MARC0080197 the genotype of SNP site is CC;     -   (103) H3GA0050489 the genotype of SNP site is TT;     -   (104) ALGA0097281 the genotype of SNP site is TT;     -   (105) H3GA0050490 the genotype of SNP site is CC;     -   (106) ALGA0097282 the genotype of SNP site is TT;     -   (107) ASGA0079089 the genotype of SNP site is CC;     -   (108) INRA0055354 the genotype of SNP site is GG;     -   (109) ASGA0079091 the genotype of SNP site is TT;     -   (110) ASGA0079090 the genotype of SNP site is CC;     -   (111) H3GA0050491 the genotype of SNP site is AA;     -   (112) MARC0056017 the genotype of SNP site is TT;     -   (113) MARC0055759 the genotype of SNP site is AA;     -   (114) H3GA0050495 the genotype of SNP site is GG;     -   (115) ALGA0097291 the genotype of SNP site is GG;     -   (116) ALGA0097290 the genotype of SNP site is GG;     -   (117) CASI0006683 the genotype of SNP site is GG;     -   (118) ASGA0079098 the genotype of SNP site is CC;     -   (119) ALGA0097297 the genotype of SNP site is GG;     -   (120) H3GA0054426 the genotype of SNP site is TT;     -   (121) ALGA0098112 the genotype of SNP site is GG;     -   (122) MARC0089391 the genotype of SNP site is GG;     -   (123) ASGA0089892 the genotype of SNP site is AA;     -   (124) ASGA0097792 the genotype of SNP site is CC;     -   (125) MARC0046857 the genotype of SNP site is GG;     -   (126) MARC0077194 the genotype of SNP site is AA;     -   (127) ALGA0108769 the genotype of SNP site is CC;     -   (128) H3GA0050799 the genotype of SNP site is CC;     -   (129) ALGA0098120 the genotype of SNP site is CC;     -   (130) ALGA0098123 the genotype of SNP site is GG;     -   (131) ALGA0098128 the genotype of SNP site is GG;     -   (132) ASGA0079719 the genotype of SNP site is CC;     -   (133) ASGA0079728 the genotype of SNP site is GG;     -   (134) ASGA0080429 the genotype of SNP site is CC;     -   (135) MARC0052755 the genotype of SNP site is GG;     -   (136) ALGA0098918 the genotype of SNP site is GG;     -   (137) ASGA0080432 the genotype of SNP site is GG;     -   (138) ALGA0098922 the genotype of SNP site is CC;     -   (139) INRA0056206 the genotype of SNP site is GG;     -   (140) INRA0056207 the genotype of SNP site is CC;     -   (141) ASGA0080435 the genotype of SNP site is CC;     -   (142) MARC0068495 the genotype of SNP site is GG;     -   (143) ASGA0080436 the genotype of SNP site is CC;     -   (144) MARC0003370 the genotype of SNP site is CC;     -   (145) ASGA0085659 the genotype of SNP site is CC;

If more than one of the samples to be tested do not satisfy all the standards of the above (1) to (145), the pig population to be tested is a candidate for non-Wuzhishan miniature pig inbred line population.

In the method, samples to be tested with statistic significance are obtained by randomly sampling from the pig population to be tested.

In the method, more than 16 pig samples to be tested are obtained by randomly sampling from the pig population to be tested.

The pig to be tested can be Wuzhishan miniature pig inbred line or Hainan Wuzhishan pig.

The Wuzhishan miniature pig inbred line can belong to any one of F₂₀ to F₂₂ generations.

The Wuzhishan miniature pig inbred line can belong to F₂₀ generation or generations later than F₂₀ generation.

In the method, specific chips can be employed to test genotypes based on 145 SNP sites of the pig to be tested; the specific chips are nucleic acid chips in which single-stranded DNA molecules shown as Sequences 1-145 in the sequence listing are immobilized at different points, respectively.

THE BEST MODE FOR CARRYING OUT THE INVENTION

The following examples are convenient for better understanding the present invention, but not to limit the present invention. Unless otherwise specified, the experimental methods in the following examples are all conventional methods. Unless otherwise specified, the experimental materials used in the following examples are all commercially available from conventional biochemical reagent stores. The experimental animals include 48 Wuzhishan miniature pigs inbred line, of which 16 for F₂₀ generation, 16 for F₂₁ generation, and 16 for F₂₂ generation, as well as 16 non-inbred line Hainan Wuzhishan pigs.

Wuzhishan Miniature Pig Inbred Line:

Li Kai, Feng Shutang, Mou Yulian, Yang Shulin, Han Jianlin, Liu Lan, Yuan Xinxu, Guo Yong, Study on Genetic Regulation of Microsatellite Loci Gene of Three Inbred Families of Wuzhishan Miniature Pig, Scientia Agricultura Sinica, 2012, 42 (5): 1751-1760.

Hainan Wuzhishan Pig:

Hou Guanyu, Wang Dongjin, Guan Song, Rong Guang, Huang Xianzhou, Detection of Genetic Quality of Small Population of Wuzhishan Pig, Acta Ecologiae Animalis Domastici, 2007, Volume 28, Issue 6: 44-47.

Example 1, the Establishment of the Method

The inventors of the present invention found that there are 145 SNP sites when genome researches are made on Wuzhishan miniature pig inbred line and non-Wuzhishan miniature pig inbred line, and Wuzhishan miniature pig inbred line and non-Wuzhishan miniature pig inbred line can be identified according to their genotypes. The genotypes of 145 SNP sites of Wuzhishan miniature pig inbred line are all homozygous types (genotypes described in Table 1). The genotypes of 145 SNP sites of non-Wuzhishan miniature pig inbred line are all heterozygous types.

In the 145 SNP sites, 12 SNP sites are located at first segment of chromosome 1, 16 SNP sites are located at 15th segment of chromosome 1, 14 SNP sites are located at 50th segment of chromosome 1, 16 SNP sites are located at 78th segment of chromosome 1, 15 SNP sites are located at second segment of chromosome 2, 14 SNP sites are located at 12th segment of chromosome 2, 13 SNP sites are located at 19th segment of chromosome 2, 20 SNP sites are located at fifth segment of chromosome 18, 13 SNP sites are located at 14th segment of chromosome 18, and 12 SNP sites are located at 20th segment of chromosome 18.

Based on 145 SNP sites, the inventors of the present invention designed 145 probes (the binding domain of probe with genomic DNA is adjacent to SNP site, and located upstream or downstream of SNP site, therefore, genotype of SNP site can be obtained via terminal extension).

As for names of 145 SNPs, their located chromosomes, their positions on the chromosomes, the genotypes of Wuzhishan miniature pig inbred line, source types of SNPs, nucleotide sequences of 145 probes, and source sequences of SNP on genomic DNA, see Table 1.

TABLE 1 Genotype of Wuzhishan the positions miniature Source SNP on the pig inbred type of chromosome name chromosomes line SNP probe Source sequence 1st 1 M1GA0025062 2687123 GG [T/G] TTTGGTCTAC NCTGGAGACCTGCTGCA segment AGAACGAGA TTAACGGCATTCGGCCG GTCTCGCGTC GTGCTGGGCCTGGGCCT GGGGTCTGAG GCTCTGCCC[T/G] CACG CATCTTGCGT CAAGATGCTCAGACCC G CGACGCGAGACTCTCG TTCTGTAGACCAAA GA AATCTACA 1 DRGA0017627 2737789 GG [A/G] TCTGATACAA CCTCATTTCT TCTGATAC ACAAATTCCG AAACAAATTCCGACTT ACTTGTGGAA GTGGAATTGTAAATATT TTGTAAATATT TGTCGATTC [A/G]TCTTT TGTCGATTC CTTTTAAAGNNNNNNNN NNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNN NNNNNN 1 H3GA0056137 2760895 CC [T/C] TGGTGTTGGC NNNNNNNNNNNNNNCT TGCGCGTGGG GCGAACTACTCTTAAGC AGGGAGGTAT AGAACAGGGAGGGGCC CTTAGAAGCA AAGTCCAAGAG[T/C] TTA AACGGGATAA TCCCGTTTGCTTCTAA GATACCTCCCTCCCAC GCGCAGCCAACACCA C GGGGAAGCG 1 MARC0039661 2780208 AA [A/C] ACCAGGGGAC TCTGGTGCAC ACCAGG AAAGGAAACC GGACAAAGGAAACCTG TGTTTCCTACC TTTCCTACCGTTCCATT GTTCCATTACG ACGTCAGTGTG [A/C]AA TCAGTGTG TTAACAAACACCTGAAA ACATCACAGAATCCTTTT GAGTAAGAAAACCCAA AACTTNN 1 ASGA0085025 2788885 TT [T/C] ACAACAAAAA AGACCAAAAA ACAACA AGAAAGCAG AAAAAGAAAGCAGGCC GCCAAAAAAG AAAAAAGAGTTCACAC AGTTCACACT TCTACAAGGCCA [T/C]G CTACAAGGCC TAGGCAAAAATCCTAGA A AAATGCAGCTGAGACAC GCAAAGGGGATAGGCAA GGCACACA 1 ASGA0098570 2789574 GG [A/G] CGAATCGAAG NNNNNNNNNNNNNNNN AAAATACAGA NNNNNNNNNNNNNNCT CTCTCTGTAGT GGAAGGCATGTATCTAAT TCCAGCACCA CGTTAATGGT[A/G] CTTC AAGGAGAAG TCCTTTGGTGCTGGAA CTACAGAGAGTCTGTA TTTTCTTCGATTCG CCT TTTTGTA 1 MARC0063358 2829500 AA [A/G] CTGCTTTACTC CTGACTCCAGGCACCGG AGTCCTTCTT GATAAGAGCAGCTCTGT GTGTCGCGTC TGTCACAGAACTTGTCA TGTCATCGTT TCTACCGGA[A/G] GAGC GTGTAGCTC TACACAACGATGACAG ACGCGACACAAGAAGG ACTGAGTAAAGCAG AA GGAACAAG 1 ASGA0085437 2835242 GG [A/G] TCGTCGTAAG CGTCGGGCCC TCGTCGT ACTGTCGCAA AAGACTGTCGCAAGAC GACTGTCGCA TGTCGCAGTGTCGCAG GTGTCGCAGT TGACAGCACCC [A/G]GG GACAGCACCC GAGNNNNNNNNNNNNN NNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNN NNNNNNNNNN 1 MARC0096709 2840543 CC [T/C] CCCACTGAGC AAAAGAAGCT CCCACT AGACAAATCA GAGCAGACAAATCAGC GCCTTAAACA CTTAAACACTGCTGAC CTGCTGACCT CTTAAACATCAG [T/C]T TAAACATCAG CGATATTCTAATATTTTCT TTAACCACATGCACTTTT AGGAGTAATACACCCAT GCACT 1 H3GA0052387 2908940 GG [T/G] GGGTCCCCAG ACGTGGCTCG GGGTCC CAGCGTCTTT CCAGCAGCGTCTTTTT TTATTTGTATT ATTTGTATTAGTGGTGT AGTGGTGTCT CTGCCAACAAG [T/G]GG GCCAACAAG CCAACGTGTCGCCACTG GCACTTCTGATGACACG CACAAGTTGGTTCCCAC TGCTGTG 1 ASGA0000264 2950020 TT [T/C] GGCTGGAGGA GGGCCTTCTGCCACAGC ACATGGATTT ACTGCCACGCCCCCCAC GGAAGCAGA AGGCGCCCTGCTGTCCA AGCTCGGATG GCTTGGGCG[T/C] CTCT GAGATGCAGA GCATCTCCATCCGAGC G TTCTGCTTCCAAATCCA TGTTCCTCCAGCC CCG GGGTCCC 1 ALGA0000195 2965787 TT [T/C] CTCTTCCAGG GTGTCCGCGCCAACTCA TAAGATTCATT CGATTCGCTATTGATCAG TACACCGAAT GTGTCTCACGGCTCTGC CCATTTTCCGC GGGGCAGA[T/C] GCTAA AGCTTAGC GCTGCGGAAAATGGAT TCGGTGTAAATGAATC TTACCTGGAAGAG ATG CTGACGA 15th 1 H3GA0001444 38635057 AA [A/G] GGTATCCCTTT GAGACTACAA GGTATCC segment AATATAGGCG CTTTAATATAGGCGTTC TTCCTAACCT CTAACCTGGATGTTTTT GGATGTTTTT GAGTTGAAC [A/G]TAGG GAGTTGAAC CCACTGGAGAATTTGTA CGTGAAAAAAATTCTAT CTTACTTCCACTAATTTC CAGT 1 ASGA0002293 38700672 CC [T/C] GGCCATGCCT GTAGCAAAGTGGGTTAA GCAGCATATG GGATCTGGTAATGCTGC GAAGTTCTTG AGCTGTGGCATATGTCCC GACCAGGTAT AGCTGAGG[T/C] TCAGA TGAGATCTGA TCTCAATACCTGGTCC AAGAACTTCCATATGC TGCAGGCATGGCC AAA AAAGGAA 1 INRA0001752 38719724 GG [A/G] TAGGTAGATA ATTTTATCTG TAGGTAGA GATAGACAGA TAGATAGACAGATAGTT TAGTTAGATAG AGATAGCAGACAATAG CAGACAATAG ATACAGAAA [A/G]ATGG ATACAGAAA TGAAAAACCCTATCAAA ATTAGAAGGAAAAGAAG TGAAAAAATATGATTCAT TCCA 1 H3GA0001445 38742311 GG [A/G] TGGGAATACA TTAACCACTGCGCCACG CAAAAGAATT ACGGGAACTCCATCAGC AAATCAACAC TTATTTATTCTTTAGATAA CTTTCACAAT ACCCAGA[A/G] TTGGCC CACAGGCCAA TGTGATTGTGAAAGGT GTTGATTTAATTCTTTT GTGTATTCCCA TCCTGA GAAT 1 ALGA0002595 38758520 TT [T/C] TCAAGAAGAA ATGTTCAGATGTTCACAA TTTCTAGCTG TTTCCATGTGAAAAGTTT AGATGAAAAG ACTATGGCTTATAGTGAA AGTTCTCCTC TGACAT[T/C] TTTTACTT CAAAGTAAAA TGGAGGAGAACTCTTT TCATCTCAGCTAGAAA TTCTTCTTGA ACTTGAT CTC 1 ALGA0002600 38781031 GG [A/G] GATATACAGTA GGCAGAAGTTGTTTTGT GGAAAGATAT ACATCATTGGATCAATTG GTCAGCTCCC AAAAATTGTCCACAGAT TCTCCCTCAC AACTGAAT[A/G] GTTAG ATAACTAAC TTATGTGAGGGAGAGG GAGCTGACATATCTTT CCTACTGTATATC ATAC TCAGAA 1 DRGA0000563 38827035 CC [A/C] GATTCTGAAC AGCATGTTAGCATCACAT TTGAAACTCT AGAAAGAACAACATGA CACTTCTTCCT AAAGAGAATTTTAAATT AAGATAAGCA GGTCTTTGR[A/C] CATGT AACTACATG AGTTTGCTTATCTTAGG AAGAAGTGAGAGTTTC AAGTTCAGAATC ATTTC AGTTT 1 DRGA0000565 38844193 CC [T/C] GGAGTCATAT AAGGGCTAGA GGAGTC GTCTATCAGTA ATATGTCTATCAGTAAA AAGAGTCAGC GAGTCAGCTAATACTTT TAATACTTTAA AAACTTTGCC [T/C]GCC ACTTTGCC ATTGGTCATTACCTTTGT TGTTAGACCTTCCTTAAA GAATGGCTAAAGGTAGT TCTT 1 INRA0001761 38870043 AA [A/G] TTCTGGCACA TTTTATGTGA TTCTGGC AATGTGCTAC ACAAATGTGCTACTTTT TTTTATATCTT ATATCTTTTTCCCATTT TTTCCCATTTT TAAAACTTT [A/G]CATTT AAAACTTT GTATTTGTTTGTTGTTAT TTAATAATTCTTTATATAA TTAGGATATTTACATTCC 1 MARC0104045 38873519 CC [T/C] GAGCCACAAT TTCCAAAAAATACTTAA GGTAACTCCT AAACTCGCCTCCCCCAA CTTATAGCAAT ATATTACTTTTCCAAAAA TTTTAAATACC AGAAAAAA[T/C] ATTTT ATGAAAAT CATGGTATTTAAAAATT GCTATAAGAGGAGTTA CCATTGTGGCTC AGCA GTAACA 1 ALGA0002601 38889713 CC [T/C] CTTCCTTTTTC CTGAGAATTCTCTCATTG CTTTTCAAGA CACCCCACCTTGGTGAA GCTTTGCTGC GCTCATCAAGCACGGGA CCAAGACAGG GATGATCA[T/C] CTCTGG AATCCAGAG ATTCCTGTCTTGGGCA GCAAAGCTCTTGAAAA GGAAAAAGGAAG GAGT TCCCGT 1 DRGA0000569 38910676 AA [A/G] TTGGTCTTTTT ATTACCTGTTACTTTTAA TGTAGCCTTC GATAAAAATTTACATTTG CTTTTTTCTGC AAACCCAAGACAAATAC CTTTTAAATAC TGTGAAA[A/G] CACCAC TGTGGTG AGTATTTAAAAGGCAG AAAAAAGGAAGGCTAC AAAAAAGACCAA GCTG GGGCAA 1 ALGA0002608 38931921 TT [T/G] AGGGGAAGA AAATCCCAGC AGGGGA CATGGTTGTG AGACATGGTTGTGGCT GCTTGACCTT TGACCTTGGAAAGATG GGAAAGATGA AGCTAAAGGTGC [T/G]G GCTAAAGGTG AGGAAACCATAAATTCA C ATTTGCAATAATAACAAA AATAAATGTTGTTTACAC TTTGCC 1 DRGA0000568 38946998 CC [T/C] ATCACATACAT GCAGTATATC ATCACATA ACTGAAAAGT CATACTGAAAAGTGAT GATGGAACAT GGAACATATCTTGAAA ATCTTGAAAC CCAATTTGAC [T/C]TAGT CAATTTGAC AACTTCATATTTAAACAT AATTAGAAACACAAAGA TTTATTTATCCCATTAATT GA 1 ALGA0002604 38953492 GG [A/G] CAGTAAAGTG GTTTTGCTGT CAGTAAA CTTCAGAGCT GTGCTTCAGAGCTTTG TTGGTAATTTG GTAATTTGGGCATCCT GGCATCCTTGT TGTATCACCTT [A/G]ATT ATCACCTT GTACTCTCATGTTAATGA AGTCAGCAACAAACATC TTACAGTATAATCTCTTA CATA 1 MARC0048118 39019040 TT [T/C] CAATGGAGGT TAYAAAGATTCTTYATTG AATAAGAAAT AAAGTAGGKGGGAAAA GGTTAGTTCTA ACACAACCCAAAAGAA TATGTACTTTG ATATTTTTGG[T/C] TTTG AGTGCAAA CACTCAAAGTACATATA GAACTAACCATTTCTTA TTACCTCCATTG CTGCC GCATC 50th 1 ASGA0004229 120634567 CC [T/C] AAGGCACTTC CAGCCAAAAT AAGGCA segment ATAGTTAATAG CTTCATAGTTAATAGCT CTATTAGAATC ATTAGAATCCTAGAGCT CTAGAGCTGC GCATAGAGAA [T/C]ATA ATAGAGAA AATCCACAGGAACTTTA AGCTCACTCTCCTCTCTT GTCTTTTATTAGAGAATC TACA 1 MARC0007088 120722033 CC [T/C] AACAGTCCAC TCACAGGTGAGGAAGG CAGACAAGCC GAGGCTGCAGGGCTCAC TCTGAAAGCC GACTTTAGCCACACACC CAGGGCACAG TTGTGTGAGG[T/C] ACA CCACCACTGT GTGGTGGCTGTGCCCT GGGCTTTCAGAGGCTT GTCTGGTGGACTGTT C CCCATAAGT 1 MARC0000061 120816036 GG [A/G] AAGACGTGAT GCTGTTGGGA AAGACG GCAGCAAGCT TGATGCAGCAAGCTCT CTGTGATGTA GTGATGTAACAGGTAC ACAGGTACCC CCCACTCTGCTG [A/G]C CACTCTGCTG TTTTGGGCTTCGGTGATT GGCTGCTACCTGCTTATA AGGTGAACAAAAGATAT TTGCTG 1 MARC0094747 120855165 AA [A/G] TTCAGATCTC ACAAGAGCCT TTCAGAT AGTCTGGACA CTCAGTCTGGACAGGG GGGCAGTGTG CAGTGTGTTCTTTTTAT TTCTTTTTATG GATAATGGGC [A/G]CTG ATAATGGGC AGGCTCAGAGGTGGACA TTTGTATAACTCTTGAGG TCATTGGTGTCATTGGAC AGGG 1 MARC0007969 120894943 AA [A/G] CTGAGGATGG TTTTTGCCTC CTGAGGA TTTTATGCCAA TGGTTTTATGCCAAGA GAGACTCCAC GACTCCACAGTCAGCT AGTCAGCTTA TAATAACCTTA [A/G]GC ATAACCTTA GACTCATGATTGAGAAG ATACGTTTATTGTTGAAA ACTGTAAAAACAGAATA CATTCC 1 ASGA0106092 120908439 CC [A/C] AAATGGTTCT NNNNNNNNNNNNNNNN GACCCAAAAG NNNNNNNNNNNNNNNN AGAGGACTGT NNNNNNNCTGGTGCCCT TCTAAGCAAC GTCTGGCCAAT[A/C] CCT CATGTGCAGG GCACATGGTTGCTTAG AACAGTCCTCTCTTTT GGGTCAGAACCATTT C CAGTGCAAG 1 ALGA0119806 120951068 GG [A/G] TGGACTTCCC NNNNNNNNNNNNNNNN AATGCCTGCC NNNNNNNNNNNNNNNN TTGTCTCTCCT NNNNNNNNNNNNNNNN ATCCCAGAAT NCTGTATGGTCA[A/G] CA CCCTCAGTG CTGAGGGATTCTGGGA TAGGAGAGACAAGGCA GGCATTGGGAAGTCCA GGGGTCGAGA 1 INRA0003745 121128221 AA [A/G] ACTGCAGCAA TTCCCAGAGCAGACTCG TAAGTGCAAC TTGGACTCAAGACTCGT TGTCACTTTG TGGACTCAGGATGAGTT TTCTGTGGAA CTCATAACC[A/G] CCAG AATGCCCTGG GGCATTTTCCACAGAA CAAAGTGACAGTTGCA CTTATTGCTGCAGT TTC CCAACAA 1 INRA0003746 121128357 TT [T/C] CATTTGTTCTT TTGCCTAACATGCAATCA TGGGGAAAAG TTAGAACTTTATTGCCTT ATTGGACAGA GGATCTAAGGCTTTTCA AGAGATCCAT CTGCTTC[T/C] GGAGAA GTTTTCTCC AACATGGATCTCTTCT GTCCAATCTTTTCCCC AAAGAACAAATG AGAG ATGAGT 1 ALGA0005490 121307042 CC [A/C] TCTCTTGCAC TGGTATTCCA TCTCTTG AAGGGGATAT CACAAGGGGATATTCT TCTATTGAATT ATTGAATTAATGGCCCT AATGGCCCTG GGAAGGACAA [A/C]AG GAAGGACAA GTAGCCATGACCCACCA GCCATGGAGCTGTCCAC CCTGGAAGCACCCTCTT AGTCAGG 1 H3GA0002538 121356509 CC [A/C] CACATTAAGC CCTCTGTGAC CACATTA CTTATGTTTTA AGCCTTATGTTTTACTT CTTACCTCGG ACCTCGGAAGCATGAG AAGCATGAGC CCATGTGATA [A/C]CTG CATGTGATA GGAGGATGGTGTAACAG GATGAGGCCCTGTGCCT GGAACACCCAAATCTAC CCAGAG 1 INRA0003749 121441133 CC [T/C] ATCTGAATAC AGTTTTTCATTTTAACTT AGCCAACGTT GGATTTGTATCCAGATGC CAGTGATACC TACTGAGGGGGATTCCT ACACCATCCA AYACTGA[T/C] ATGTCCA CTTTGGACAT AAGTGGATGGTGTGGT ATCACTGAACGTTGGC TGTATTCAGAT TGTCTA TCTC 1 H3GA0002539 121478005 GG [T/G] ATCAATTTGTA CATGGATATACAGAAGTT CCACGAGTGA GCAAAAGTCCCTGTGGC GTTGGAATGA CAGACCTGGACGGCGGC CTGGCATTAG TATATAAT[T/G] TGTCGA GATTCGACA ATCCTAATGCCAGTCAT TCCAACTCACTCGTGG TACAAATTGAT GCAACT GAAG 1 M1GA0001099 121626075 CC [T/C] AAACTCACCA ATTCGGAAGT AAACTCA GGGCAACATC CCAGGGCAACATCTTC TTCCATGAAG CATGAAGCTCATGTAA CTCATGTAACT CTTTAATGCCT [T/C]GTC TTAATGCCT CTGGCATTGTTTCCCCA GCCGGTACTGGCCCCTC TGCGTGGCCTCCTGGAG GCCAGG 78th 1 MARC0111831 225656477 AA [A/C] AAATACCTTC CCTAGGCAAC AAATACC segment CCGTTCTCCC TTCCCGTTCTCCCATTT ATTTCTAATAT CTAATATAAGACAGGG AAGACAGGGA ATGAGGATAC [A/C]NKK TGAGGATAC WAAANNNNANNNNNNT TTTAAAANGCCCANGAN GNCTTTCTTTTATTGAAG TATATT 1 ASGA0005566 225713764 CC [T/C] AATATGACTG ACTTCAAAAT AATATGA AGTTAGGTAC CTGAGTTAGGTACTTG TTGGAGGGTG GAGGGTGAAGGATAAA AAGGATAAAA AAAGATACAGA [T/C]TA AAGATACAGA GCTATGGAATGATAACCA TTAAGGAAGAGATATCTA TAACAATTTACTACATCT TCAA 1 ALGA0007578 225761304 CC [A/C] CTTGGGTGCT ATAGGAAGGA CTTGGG CTTGAAAATC TGCTCTTGAAAATCCC CCAATTCAATT AATTCAATTCAAACCC CAAACCCAGC AGCTGTTCTGAT [A/C]A TGTTCTGAT AAATTCTGGAGTTAGTA CTAAGGATATGGTCCTGC TCACAGACACACTGGCA TTTCCCA 1 ASGA0005568 225825860 CC [T/C] TGATCTGGCA TCAACTTCAGACTACTTT TTTGAGGACT TGGCCCTCAGTCCAAAT GTTGTATCAGT TCAAACCCTATTTGTGA TTGAAAGTAA AATTGCTC[T/C] CAAGCT GACAGCTTG GTCTTACTTTCAAACT GATACAACAGTCCTCA AATGCCAGATCA GCTCC AGAGC 1 INRA0005765 225854493 AA [A/G] CCCTCCCCCTA CTCCCAATGATAATAAGC TATATATTTAA AGTTACCAAGAGCTAAC AATATAGATGT TGTTGTTTTCATATTTCTA TGCTTAATATC TTTTTT[A/G] TGTTCTGA AGAACA TATTAAGCAACATCTAT ATTTTAAATATATATAG GGGGAGGG TTTTCTGT CT 1 MARC0095347 225889929 CC [A/C] TAGAGTTTGT ATCTCTCAACTTTTTCCT GCCAATCTGG TCAATCAAGACTTAATTC TTGTGCCACA TCTTATGCAAACCCACAT GCAGGGACTC TTAAAA[A/C] TGTGTAC CCTGTACACA AGGGAGTCCCTGCTGT GGCACAACCAGATTGG CACAAACTCTA GAGGC TGAGA 1 MARC0039015 225944274 CC [T/C] TGACTAAATA CCCAGATTACTAATCAGT AGGTTTGTTC [T/C] GATCTTTTTCATTA CAAATATTACA TGCCCTGTAATATTTGG GGGCATAATG AACAAACCTTATTTAGT AAAAAGATC CA GAGTGCTTCA 1 DRGA0001892 225986595 GG [A/G] TTAAGTACTT GGAATCCTAA TTAAGTA GCCAAAGAAT CTTGCCAAAGAATTAA TAAAATAAAG AATAAAGATAAGCAAT ATAAGCAATG GCTTTAAAAAA [A/G]AT CTTTAAAAAA AGAAAGAAAACAAATG AATACAAAGTTGAAAGG AAATTGTATTTGAAAAA GCAACTGA 1 ALGA0007580 226149776 GG [T/G] GCTCATTCATC GAATGTATGCTTACATAC CAACACATAC AGTCGTATCAGATGGAA ACACAGGCTA GAAGCTAGTTTCTAAAA ATCAGAAGCA CAGTTCGG[T/G] GTGGG AACACCCAC TGTTTGCTTCTGATTAG CCTGTGTGTATGTGTT GGATGAATGAGC AATT GGGAAG 1 ALGA0007583 226188042 TT [T/C] AGGAAAATTC TATCAAGGGA AGGAAAA CCTGCCTAAA TTCCCTGCCTAAAGCC GCCACATAGC ACATAGCATCATCTTAG ATCATCTTAGG GAAATAAACC [T/C]GGG AAATAAACC GCTTCTTATTCCCAGCTG ACTGTTTTCTTTGAGATC ATTGAAAAATATATATAA TTG 1 MARC0095915 226237014 CC [T/C] GCTACAGATG GCAAAGTTAT GCTACAG TATACGTAGAT ATGTATACGTAGATTCT TCTTATAACTG TATAACTGTCCAATGAA TCCAATGAAC CTAAGTGTC [T/C]AACA TAAGTGTC TGAAAGATGTTTGGTTA AAATGTTAGATTTAATTG ACAAAAAACCATACAGA TGTT 1 ASGA0005571 226278675 AA [A/G] CCATGTAGTTT TCACACATTG CCATGTA TCAGTCCACT GTTTTCAGTCCACTGT GTGAAATTTT GAAATTTTAACCTTTTT AACCTTTTTC CGTATGTCTA [A/G]CCA GTATGTCTA ATCTGGTATCCCAGCTTT GGAGGTGGGGGTATTCA GAGAAGTGGGCATCTGA TTCAA 1 MARC0014134 226300834 AA [A/G] CAATCATTTCA C CAATCATTTCATATAC TATACCAGTGT CAGTGTGCAAATATAAT GCAAATATAAT ATGCAAGACCAAAAAA ATGCAAGACC A [A/G]KWACATATTGAGT AAAAAAA CTCTATTATGCCATATGC CGTGTACCATATATTACT TCATTNAANNN 1 MARC0024233 226319468 AA [A/C] AACCTGTTTC AGTGTTTTGT AACCTGT ACTCTTGTCG TTCACTCTTGTCGATG ATGCAGCCAA CAGCCAAGTGGCAGG GTGGCAGGGA GAAGTCCTCAGC [A/C]A AGTCCTCAGC ATTTGTGCCTTGGGAAA TGG 1 H3GA0003548 226335313 GG [A/G] AGCTTAACAG AGAGGGGAGA AGCTTA GTGGCTCTGG ACAGGTGGCTCTGGAG AGGAAACACA GAAACACACTTGGTCT CTTGGTCTGA GAGATATCACGG [A/G]G GATATCACGG GCCTCCTCTTGGCTTGTT GCAAGGACTTGACTTTC CTTCTGTGTAAGACAGG AAGCCTT 1 ALGA0007591 226408018 CC [T/C] ATAAAAAATG AGCAGTAGAAAAAGCA AGTTGGTGAG CCAAAACAGGAGAGTC TTTGTACAAG AGAGAACAGGGTGTCA TCACTCAGCT GTTCCATTCTTA[T/C] CA ACCCAGTGTG CACTGGGTAGCTGAGT GACTTGTACAAACTCA CCAACTCATTTTTTAT C ATGTAGGAA 2nd 7 MARC0000195 12606764 CC [T/C] GGAAGCCAAT AAATATGGGCTTCTTGG segment ATGTAAGATA GATAACATTCACGAAGA GTAAGGTGAC TGCTGAAGGAAGGCTTC TCATCTTTCAT AGCATGCTT[T/C] GCTGC TGGTGCAGC ACCAATGAAAGATGAG TCACCTTACTATCTTAC ATATTGGCTTCC TCTTC AAAAT 7 ALGA0038697 12641640 CC [T/C] CAAAGGGTCC CCTGTGTCCTGCTCAGC TTTCTCCCCA ACATGACTTTTGGTGTG GTAGAGCCCC TCGACTTCAGAGCTGCG TAATGAAGAA TGGGATGGA[T/C] CCAA ATGTTCTTGG GAACATTTCTTCATTAG GGGCTCTACTGGGGAG AAAGGACCCTTTG ACA GTTAAGA 7 H3GA0020000 12679908 CC [T/C] CAGTAACAAA CCTAAGGCTA CAGTAAC TTACCACAAA AAATTACCACAAACTT CTTGGTGGCT GGTGGCTTGTAACAAT TGTAACAATA AGAACCGTGTT [T/C]TC GAACCGTGTT TCATTCTTTTGGAGGCC AGAAGTCTAAAATTTTTA TCACTGGGCTAAAATCA AGGGTC 7 ALGA0038703 12710295 CC [T/C] ACAAATATCTC ATCACAGCCT ACAAATA ATGCTCATCTT TCTCATGCTCATCTTGA GAGCCCTCGT GCCCTCGTATGTGAGG ATGTGAGGCA CAAAGTGTGG [T/C]GCC AAGTGTGG CAGGTGTCTTTGAATAG CTATATAATTAAGGTTTCT ATTTTTTAAATCTACCTT CAT 7 H3GA0020002 12731286 GG [A/G] AATATTACAAC TGTTGACTGATTACTTGT TAGTTTGACTT GATTCTTTTTTTTTTTTTT GCTAAGTGAG TTTTTTACTGCCTGTACT AGCCAATCCA CCCTT[A/G] GGGCCTTG CAAGGCCC TGGATTGGCTCTCACT TAGCAAGTCAAACTAG TTGTAATATT ACTACAA CCA 7 DRGA0007138 12743235 CC [T/C] TTCCTTATATA AATGTTCAAG TTCCTTA AAACAGTATA TATAAAACAGTATAGTA GTATCTGCATA TCTGCATATAACCTATA TAACCTATACA CACGTCCTC [T/C]TATAT CGTCCTC ACTTTAAATAATCCTAGA TTACTTGTAATACCTGAT AGAATATAGTGCGAATAT G 7 ASGA0031284 12781950 CC [T/C] TCCAAGTCTC GGCTTTTCTCTACTTTAA AGCCGACTTG AGGCTAGAAAGCTATAA GGAAATGGAA ACTACATTTCCCAGACT ATCCCCAGAT GCCTTGTA[T/C] CTGAG GATACCTCAG GTATCATCTGGGGATTT CCATTTCCCAAGTCGG CTGAGACTTGGA GGGT AGAAAT 7 ASGA0031285 12812114 AA [A/G] GGTGAGTCAC GAGCTGAAGT GGTGAG TCAAATGAGA TCACTCAAATGAGAAC ACAAAGGAGC AAAGGAGCAGGATGGG AGGATGGGTG TGGAGCTAGGAG [A/G]C GAGCTAGGAG AGCAAAGAGGAGCTGG AACTTCCCAGCTTGCGG GTCAGGACCCAGCAAG GAAGGGACTC 7 MARC0025042 12829776 GG [A/G] ACACATCACA CTGTTAGCTGAAATCTTA GAAGGGAGA GTTTTTAGACTATGTAAT AATGGAGATAT GTGGAATTCATCAGTTA AGGATGAGGA AGAGGGA[A/G] GGGGT CCTGAACCCC TCAGGTCCTCATCCTAT ATCTCCATTTCTCCCTT CTGTGATGTGT AAGGAT AAGS 7 H3GA0020006 12871039 TT [T/C] AAAGTAACTG ACACTGGCATCTGCATTT CCGAAGGACA AAATTTGATGACTGGCA TGAACTAATG ATGTTGAATACTTTTCCT AAAGAAATAA GTGTTTT[T/C] GGGGCC AAAGGGCCCC CTTTTTATTTCTTTCAT TAGTTCATGTCCTTCG GCAGTTACTTT ATGAGG TGTT 7 ALGA0038729 12891883 CC [T/C] TTCTGGCCAC CTGTGTTGGT TTCTGGC ATGTACAGTT CACATGTACAGTTCTG CTGTGAGTCA TGAGTCATCCTGGCTG TCCTGGCTGT TGGCCAGTGAC [T/C]GG GGCCAGTGAC CTTTGGGAGCTGGGTCC CATAGACCAGCCCCCAG GCCAGCCCAGGGTCTGG CTCATGG 7 ALGA0038731 12918213 GG [A/G] TGCTTTGAAT TGGTTTTAGT TGCTTTG CCCAACCATG AATCCCAACCATGCCA CCATGACCAT TGACCATTTTGTAGCT TTTGTAGCTCA CATTTCAACCT [A/G]AA TTTCAACCT GGAGGCGCTGTCACAAA GGCGTGGATGGAACCAC GCAAAGTCCTGGACAGA ACCGTTC 7 ASGA0031321 12984109 GG [A/G] TTGATGATTG TGATGAGAGC TTGATGA AAGTTTCCAG TTGAAGTTTCCAGCTC CTCGCTGACC GCTGACCACTCTTTCT ACTCTTTCTTC TCACCTCCCTC [A/G]CT ACCTCCCTC CCTGCCTCCTCTGTTAGC ACAAATCCTGTCCTTGG TTTATGTCATTCCTTGCT CAGTG 7 ASGA0031322 13023831 GG [A/G] AAGAGAAAAT GTCTGGGGAAGAGGGG AGCAAACATG TTGGGGATGGGTAGATG TCCAAAAGTA AAACTAGGCTGTTGACA GATCATAAGC TGGGGTGATG[A/G] GGA TCCCATGTCC CATGGGAGCTTATGAT CTACTTTTGGACATGTT TGCTATTTTCTCTT TCT TTCTTTC 7 ALGA0038747 13071251 AA [A/G] TGTGGAGAAC TGTACATGTTTTTTGGTG AGTTTGGCAG TGAACGCTCACTTTCAA CTCCTCAAAA TTCTCTCGGGCAGAACG GGTCAAACCT GCTGGGTC[A/G] TGGTG AAAGTCACCA ACTTTAGGTTTGACCT TTTGAGGAGCTGCCAA ACTGTTCTCCACA GTG GCTGCAC 12th 7 ASGA0033095 43103102 TT [T/C] CGCATAGGAA CTCCTGGGACTCCCTCA segment GGAAAGCCGA CCTGCCTGCAGCTACTC GGTGCGCAGG ACAGGTCAGCTAGGCGG TTAACAGCCA CTCTGCTCC[T/C] GAGA GTCAGCTCTC GCTGACTGGCTGTTAA CCTGCGCACCTCGGCT TTCCTTCCTATGCG CTC TCATCCT 7 ALGA0040854 43127838 GG [A/G] TAGGGAATTAT CTCTAAAATACCACTGC CCAACCTAAG ACACTTGGGGATCTCCA GTAGTCTATG GAGCCATCTTCATTTCTA GAGATGCCTG ATCAGCTG[A/G] CTACA AATTTGTAG AATTCAGGCATCTCCAT AGACTACCTTAGGTTG GATAATTCCCTA GAACA ATTCA 7 ALGA0040856 43166101 AA [A/G] TTAAACAGTT AATGGGAACTCCAAAAT AATCATCTAG AACTTTTTTTAATAGAGT GTTTTATTTAT ATCTTGTGAAACTCTGTT AGTACCATTTT GCTTTAA[A/G] CAAGCT CCAGCTTG GGAAAATGGTACTATA AATAAAACCTAGATGAT TAACTGTTTAA AGGATA TCTT 7 ALGA0040857 43190587 GG [A/G] ATGCCATAGG CCAAGTTTAACCCCTGG CACCGCAATA CCTCCTTCAGTGGGTTA GCAGATCCCA AGGATCTGACGTTGCCA GCCGTACCTG CAAGCTGCA[A/G] TGTT TGACTTAACA AAGTCACAGGTACGGC TGGGATCTGCTATTGC GGTGCCTATGGCAT AA GCTGCAGC 7 ASGA0033096 43205140 GG [A/G] AGGAGTATCA CAGACTCTCT AGGAGTA TGCCTGAATT TCATGCCTGAATTACA ACACAGCTGA CAGCTGATAAATAGCA TAAATAGCAG GGGATTCAGAG [A/G]AG GGATTCAGAG AGCTGTTCTTGTGGTAC AACATTAACATACTTTGG TGCTTAACATTCTTTCCC ATTTT 7 INRA0025180 43234940 AA [A/G] AGCAAGGCAG TCCAGGAGGA AGCAAG GATGTGATGA GCAGGATGTGATGAGG GGAAGGAGCC AAGGAGCCAAGAAAAG AAGAAAAGTA TAGACTGCAGAC [A/G]G GACTGCAGAC AGGTGTGATGCATCTCT GGTAGAAGATGGGGGAA AGCTGGCTGCCTCCCCT ACGGGATT 7 ALGA0040859 43342944 CC [A/C] GCTTCCCTCA GACTCTCCCA GCTTCCC CGCTTTACAC TCACGCTTTACACCTG CTGTCCCTAG TCCCTAGCTGCTCCAC CTGCTCCACT TGGAGGAGGTG [A/C]C GGAGGAGGTG CGAGGTCTAACACACAC ACACACACACACACACA CACACACACAGGAAGGT AGCACCAG 7 H3GA0021216 43380793 CC [A/C] AATATGTTACA TTATCTTTCT AATATGTT ATACAAGAAA ACAATACAAGAAAAGG AGGGGGAATC GGGAATCCGCAACTTG CGCAACTTGG GACATTGGCA [A/C]AAG ACATTGGCA TAAGGAAACCATGGCAA CCGTAAAACGGCCCAGA TTTTCCAATTTCTATTGG CTCGG 7 ASGA0033098 43400560 CC [T/C] AGGTAAAGGT TTTATGGAAA AGGTAAA TTCTATTTGTC GGTTTCTATTTGTCTCT TCTGAGAAGT GAGAAGTCAAGTTCCC CAAGTTCCCT TGTTAGCTAC [T/C]CCCT GTTAGCTAC TTCCCGAATTTGCATTTT AAGAAGGTGGCAGAGA AGACCAGATAGAACATT TGTAT 7 H3GA0021221 43423062 CC [A/C] TATAGAATATT GATCTCAGTTCCCAGAC CCAATCTGGA CAGGGATTGAACCTGGG AAATCTGGGA CCAAAGCAATGAAAGCA GTTCCTTGTT GAATCTTAA[A/C] CACTA GGTCTAGTG GACCAACAAGGAACTC CCAGATTTTCCAGATT GGAATATTCTATA ACTA CAAACA 7 MARC0014540 43442829 CC [A/C] ACCCTCTATAT GGGGTCAGGAGGGGTG TCCCTTGAAG GGAGGTGAGAAGGTTTC TCTTATCTACG TGAGGCTCATGTCCTGG GAGACATCCT GAGAAAGAAT[A/C] ACA TTTGCTGT GCAAAAGGATGTCTCC GTAGATAAGACTTCAA GGGAATATAGAGGGT T CAGAGTTCT 7 ASGA0033103 43464853 CC [T/C] ATGGAAGTTG CCTGCGGCAT ATGGAAG CCAGGCTAGG TTGCCAGGCTAGGGGT GGTCCAATCA CCAATCAGAGCTGATG GAGCTGATGC CCACCCAACTA [T/C]GC CACCCAACTA CACAGCCACAGCAACGC CAGAGCCAAGCCTCATC TGCCACCTATACCACAG CTCATGG 7 DIAS0000557 43488849 AA [A/C] ATGTTCTTTCC ACATCACAAGCATGGTG TGGGGGTGCA CGGTCAGGGGGCTTGCT CAGCCTGTGC GGTCATTGATCATCG[A/C] TGAGGATGTG AACTACGACCACATCC GTCGTAGTT TCAGCACAGGCTGTGC ACCCCCAGGAAAGAAC AT CTACTATAAG 7 ASGA0033116 43505936 AA [A/G] CATCAGAACT TTGCTTTTCACTTGGCTT GCTGCACAGA CTGACTGGCTGGGTTGC TCTCCAATAG TGGGCTGCCTCTTCTGA CTCCAGGAGG CCTTCTCA[A/G] TTAAAC AAGAGTTTAA TCTTCCTCCTGGAGCT ATTGGAGATCTGTGCA GCAGTTCTGATG CTGG ATTGTC 19th 7 ASGA0036835 125807043 CC [T/C] CTGCCAAGGA TGCTATTGTA CTGCCAA segment GACTGAGAGC GGAGACTGAGAGCTCT TCTCAGGATTA CAGGATTATTGTATATT TTGTATATTTG TGCCCAAGAC [T/C]CTAT CCCAAGAC AGAGAACTTGAACCCAT GTCTGTCTGAAGTCAAA GATCATATTTTGGCCATT ACGT 7 MARC0031932 125822314 CC [T/C] TAAAATTGCT TATTCATTTAATAAAATA GATTATTTTGA CTCTATAAGCACATAAAT TCACTAATAAT AAATATGACTTCCTGATG GGTAATGCAA TTATAT[T/C] GAGTGACT AGTCACTC TTGCATTACCATTATTA GTGATCAAAATAATCA GCAATTTTA ATTACAGT TT 7 ASGA0036838 125837557 CC [T/C] ATCATGTAGTT CAGTCTGGTC ATCATGT AAATTCGTCC AGTTAAATTCGTCCAC ACCCAGTGCA CCAGTGCAGGTTTCAG GGTTTCAGTAT TATCTACCAAA [T/C]AG CTACCAAA CTCAAAGGATGTGAGCT GGAATTTTCTCCATAGCT CTTGGAGGGGAACTTTA GTTTGA 7 H3GA0023523 125850169 GG [A/G] ATTAACAGAA TACTGAATGA ATTAACA TGAAAGCCAA GAATGAAAGCCAATCA TCAGGGGAGG GGGGAGGCCATGATTC CCATGATTCTA TAGAGTGGCTA [A/G]AG GAGTGGCTA ACACCCAGCTCCCTCTG GTTCCTGTTTTCAAAAA GATCCAGTTTAGCTCCA GCTGCTG 7 ALGA0045460 125871919 TT [T/C] ATCCTATTTGC CATATTGTGGTAGGCAG CAGTCTCACA AAATCTAGGATGGCCCT CACTCACTGG CAGAATTCCTACCCCCC AAGGAGCTAT AGGGGTGAA[T/C] ACTT AGAGGAAGT CCTCTATAGCTCCTTCC AGTGAGTGTGTGAGAC TGGCAAATAGGAT GGA ATGTCAC 7 MARC0005927 125942941 GG [A/G] CAGGTCCTAC CAAGAGTGGA CAGGTC TGATGCAAAC CTACTGATGCAAACCT CTTCAACTTAT TCAACTTATAACTAACC AACTAACCAG AGTGACCTAAT [A/G]GA TGACCTAAT TCAATTCCAGAAAGATG TTAAGTGCAGGTGAGAG AAGAGGATTTGGGAAGA AAAATTM 7 MARC0005928 125943001 AA [A/C] CAGAAAGATG RGATCAATTC CAGAAAG TTAAGTGCAG ATGTTAAGTGCAGGTG GTGAGAGAAG AGAGAAGAGGATTTGG AGGATTTGGG GAAGAAAAATT [A/C]AA AAGAAAAATT TTTCTCTGCATAACAAA AGATCCAGTACTTTGAA ACATCCTCTGTCTAGGG AAGGATA 7 ASGA0036842 125986792 CC [T/C] AATATGGACAT TGGGGAAAACAATCACA GAGGTTGGCA AAAATTCTCCTAAGGTC GGAGAGCTAT ATAAATGAGTAGTCATTA TCTACTGTGG TTCTGAAG[T/C] AGACA GAACTGTCT GTTCCCACAGTAGAAT AGCTCTCCTGCCAACC TCATGTCCATATT CAGC TGAAAT 7 ASGA0036846 126014404 CC [T/C] GGATGAACAA TTAAGCAAGAAATAAAC TATTGTGTGTA TTCAGAAGTGTTAACGC CGTACTGGAG ACTAGAAGTTGAGGGTT AAGATTAACC TATTTRTTT[T/C] AGTAG CCAGCTACT CTGGGGTTAATCTTCT CCAGTACGTACACACA ATATTGTTCATCC ATTT GCAAAT 7 MARC0098637 126028158 TT [T/C] CACATGAAAT AAGCTGAAAG CACATG GAACTTTTAA AAATGAACTTTTAAATA ATAAAATGGTA AAATGGTATTTCCTACA TTTCCTACAAT ATGAGTTCCA [T/C]GAC GAGTTCCA TTTTTGGGMGATGTTGG GTCAAGAAGGGAGTTTC GTATCCTAATATTTTTGG GAGTA 7 M1GA0011035 126040140 TT [T/C] GATTTGATGCT GCTGCTCTGA GATTTGA ATGCCCGGGC TGCTATGCCCGGGCAT ATTGAAATTTC TGAAATTTCTTTAGAAA TTTAGAAACT CTAATGATAT [T/C]CTTA AATGATAT AGATTTTTCTCAGACTCT CTCCTTCAGTCCCAGGT TGAAGAGGGTCCTTCAA AAGT 7 DRGA0008230 126067443 GG [T/G] AATTAAAGCA TATAGTAAAA AATTAAA AAGGCATTAG GCAAAGGCATTAGTAA TAAGAGTTTA GAGTTTAAAAGGTTTG AAAGGTTTGT TGCTACAAAGT [T/G]AA GCTACAAAGT AGTGAATAAAGTGAAGA TTATCCACAGAGGAAAG AAAATATTTACAAATCAT GTATAT 7 ASGA0036855 126085856 CC [T/C] TAGCAGATTTT TATATATTCATTCTTACAC GCCTTATCTAC GAGGCATTATCGGACTA CCAATTGCCT ACAGAGTGAAAAGCAAT GTAAGCTGAG TGAGTAC[T/C] TGCCATC GGATGGCA CCTCAGCTTACAGGCA ATTGGGTAGATAAGGC AAAATCTGCTA CGATGA GTAT 5th 18 ALGA0097277 19527294 GG [A/G] ATCAATGGGT GTTCCTCATTTGTTTTTC segment TGAATATCTAA TGAGTAAATGAAAGCTT CAGGCATGTT CCTAGACCTCCTGCCAG TCTCTGTGTC GCACCAGG[A/G] AGACT CTCCAGTCT GGAGGACACAGAGAA ACATGCCTGTTAGATAT TCAACCCATTGAT GGCC CTGGGG 18 MARC0080197 19663776 CC [T/C] ACAGCTGAAT TTAATGTTCTTATCCTAG GACAGTGTAC GTATAATTATCCCATATGT AATAATACAA AAAATTTTTCACCTTTTT ACACTTAAAC TTTTT[T/C] ACTTTTCAT AATGAAAAGT TGTTTAAGTGTTTGTAT TATTGTACACTGTCATT CAGCTGT TCTCCTATAT 18 H3GA0050489 19711697 TT [T/C] GCAGCTGTAG TAAGTCCAACATCTATAG CTCTGATTTG TAACCAAAGAAATGCTT ACCCCTAACC TTTTGTTTGTTTTGAGGG CAGGAAGTTC CCGAACW[T/C] GCAGGA CATATCCTGC TATGGAACTTCCTGGG TTAGGGGTCAAATCAG AGCTACAGCTGC TGGT CTATGA 18 ALGA0097281 19783081 TT [T/C] CTTCTCAGGG CTCAATCTCA CTTCTCA CTTTTCCCTGT GGGCTTTTCCCTGTGT GTTCTTGACT TCTTGACTTTCCACTG TTCCACTGCC CCATCTCTCAC [T/C]AC ATCTCTCAC TGCTCTTTCCTTGTTCTT GACACAGCTGTCTCCCA TCCTTCCTGGGGAAGTA ATTCCC 18 H3GA0050490 19806130 CC [T/C] GCCCAGGTCC TTCGGGTCACTGGGCGG CATTGTCACC GTTCTGGTGGTGGGGCT CGAACTCGGA AGCGGCCTCAGGCTCAG GCTGGGACTC GACGGTCAA[T/C] AGGA TTCCAGTCCT CTGGAAGAGTCCCAGC TCCGAGTTCGGGTGAC AATGGGACCTGGGC TC CTTTCAGC 18 ALGA0097282 19883611 TT [T/C] CCCAACTGGC ACCAGAAGAT CCCAAC CTGGCTGAAA TGGCCTGGCTGAAATG TGATAAGGGT ATAAGGGTGACTTCCT GACTTCCTAC ACCTTACTCTTA [T/C]G CTTACTCTTA GACTGAATTGGCCCCAG CTCCAAATCCACATGTT GAAGGCCTAACCCCCAG TACGTCAG 18 ASGA0079089 19925205 CC [T/C] TCTACCTGCA AAACCCTCTCTCTACCT ACCTCTTCCTA GCAACCTCTTCCTACT CTCTAAACTCT CTAAACTCTAATAGCAC   AATAGCACTTT TTTATGCAGA[T/C]TTTT ATGCAGA TTTGCATTTACTCCTTAA TGTACAGAGCTCATGTT CTTTTGCTAAACTTTAAG CTT 18 INRA0055354 19972451 GG [A/G] GGAATGAGCT CAGCTAGCAGATTCAGC GCTACCTGGG CTAGCCTATAGCAGCGTA GACACAGCTA AATCTTCAGGTAAAAATA GCCTGAGTGA TATTAGT[A/G] GATGATA TTTTATCATC AAATCACTCAGGCTAG CTGTGTCCCCAGGTAG CAGCTCATTCC TGAGC AGATT 18 ASGA0079091 20017978 TT [T/C] CCTGTGCACT GGTTAGAGCAGTCTTCC CTCTTCCCCAT TGTGCGCTCGCTCGGAG TAGCGCAGAA GCTTGTAGCTGTGACAT AAGACCATCC CACCATCCC[T/C] GCTG ATCACCAGC GTGATGGATGGTCTTT TCTGCGCTAATGGGGA AGAGAGTGCACAGG GC CGTCTGGG 18 ASGA0079090 20060777 CC [T/C] TTCACCCACT GTGGGTGAACTTCAGAT GTATTGAGAA GTCCTATCCACGAAGCT AGTACCAATA CTTTGTGTTGGTATCAGA CCAACACAAA TGTCCTAT[T/C] CACGAA GAGCTTCGTG GCTCTTTGTGTTGGTA TTGGTACTTTCTCAATA CAGTGGGTGAA CTTCA GATGT 18 H3GA0050491 20112057 AA [A/G] TTCAAGCCGC TGGGATTGGCAGTGTCT AGTTGTGACC CTGGAGCACTGGGACGC TACGCTGCAG AGGTTCGATCCCTGGCC CTGTGGCAAT TGTGTGGTT[A/G] AGGA GCCGGATCCT TCCGGCATTGCCACAG CTGCAGCGTAGGTCAC AACTGCGGCTTGAA TC AGATCCCT 18 MARC0056017 20126280 TT [T/C] ACTAATTCCTT ATCCCTAAGA ACTAATT AGCCAGAGAC CCTTAGCCAGAGACAT ATAAACAGGC AAACAGGCACAGAGCT ACAGAGCTGT GTAGTTGCCCT [T/C]CG AGTTGCCCT CTCTGTGAGACACACAG CTGGCATGGAGGCTGAA TAAGGTCCCCRGATSTCA TGCTCC 18 MARC0055759 20304323 AA [A/C] GGAGCTTTCT CTGGTACTCT GGAGCTT CTGCAAGCTT TCTCTGCAAGCTTCCT CCTGGTCACC GGTCACCCATCACTGC CATCACTGCT TTGAGGACACA [A/C]AA TGAGGACACA AAAGAACAATGGTACAG AGATGTGTCCCTGAAAA TTAGGATTTTTGTGTAGT CTTTTG 18 H3GA0050495 20338092 GG [A/G] GAAGAATATG AAAAGAACAG GAAGAA TAAGCATAAA TATGTAAGCATAAATTT TTTAAAGGCC AAAGGCCCCTAGAGGA CCTAGAGGAA AGACAATATAT [A/G]TTT GACAATATAT TAACACTTCCATACTCAG TTTCTTTAATTAAAAAGA AAAGACTGAAAATCAAA ACTC 18 ALGA0097291 20376074 GG [A/G] CAACTCCAAA ATTTTTAATT CAACTCCA TATAACAACTT AATATAACAACTTTAAT TAATGGTCCTC GGTCCTCACTTAGCTT ACTTAGCTTTA TAGGATTCC [A/G]AATG GGATTCC GGAAAGGAATGACATAC ATACATACAATATTATTTC AGGGTTTTTTTTCTACCC CC 18 ALGA0097290 20404568 GG [T/G] CGGAGGCATA CAGGTCGCTG CGGAGG GCTTTGATCC CATAGCTTTGATCCCC CCAGCTCCGT AGCTCCGTGCAGTGTG GCAGTGTGTT TTACAGGATCTG [T/G]C ACAGGATCTG ATTGCTATAGCTGTGGTG TAGGTTGCAGCTGTGGC TTGGATTCAATCCAAGG TGCAGGA 18 CASI0006683 20450764 GG [A/G] GACAGTTTCT AGCAGGTGGA GACAGT AACCAAACTA TTCTAACCAAACTAGA GAGTAGCAAA GTAGCAAAATTTGAGT ATTTGAGTTC TCAAGTTGTAGT [A/G]G AAGTTGTAGT AAAATTAAACTATCTTTT TAGATCCTTACCTTGAAA TATTTAGTTAACTAGATA GGACG 18 ASGA0079098 20520014 CC [T/C] CATGAGTGGG GCAAGAGGTCCCAGAG CAAGAACACA AGGGGCCCAGGATTGCC CAGCTTGTTC CATCTACACTCCCCGGC CTGAGGTGAT AGGGGACACT[T/C] GCT GACTGGAAGC TCCAGTCATCACCTCA GGAACAAGCTGTGTGT TCTTGCCCACTCATG AT CAATGTGC 18 ALGA0097297 20545597 GG [A/G] GCCTTTGAGT CCCGCAACCTCACAGTT TTAGGAGCCA ACCAGTCAGATTCATTTC GCTATATGGA CGATGCACCACAACAGG AAAATCTAAA AACTCCTC[A/G] TGCTG TGTCTCAGCA AGACATTTAGATTTTTC CATATAGCTGGCTCCTA AACTCAAAGGC ACCTT GTTGA 18 H3GA0054426 20806040 TT [T/C] GGAGGGAGG GTAGGGAACA GGAGGG GATCAGGACC AGGGATCAGGACCTAA TAAGTAGGGC GTAGGGCCATTCTGGA CATTCTGGAC CAAGTGGGCAAA [T/C]G AAGTGGGCAA TTGTCCAGGGCAAGTGG A GAAGCCCGGAGNNNNN NNNNNNNNNNNNNNNN NNNNNNNNNN 14th 18 ALGA0098112 43103162 GG [A/G] TCAAACATCC GCAGCTGGGAGGCCTGA segment TTATTTTCTTT GGCATAAGTTGGCAGAG TCTATTTTTCT GTGATGGAAAGGGATGG CTCCTATACTC AAGCTRGAA[A/G] CCGG TAGCCGG CTAGAGTATAGGAGAG AAAAATAGAAAAGAAA ATAAGGATGTTTGA ATC CCATCAG 18 MARC0089391 43127872 GG [A/G] TCTGGCAGCC TCTTCTTCCCATTGTCTC GGCTATAGAG AGCTRTTGCCCTGGTGG GGATAGGAGG AGCCCATCTTCATCTCTA GCAAGGAGCT GCAGCCC[A/G] CAGACC CAATGGTCTG ATTGAGCTCCTTGCCC TCCTATCCCTCTATAGC CGGCTGCCAGA ACGAA TT 18 ASGA0089892 43229113 AA [A/G] TGGAGGTGAT GGCTTCTCTG TGGAGGT AGTCCAGCCA GATAGTCCAGCCAAGC AGCATTGAAG ATTGAAGGACGGAAGG GACGGAAGG GGGTTAGGACA [A/G]GA GGGTTAGGAC GNNNNNNNNNNNNNNN A NNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNN NNNNNNNNNN 18 ASGA0097792 43232145 CC [T/C] GGGGCCTCTA NNNNNNNNNNNNNNNN AGCCACCTGT NNNNNNNNNNNNNNNN CACTCATCCT NNNNNNCTGTGAACAA CTTTCCTGGC CCACAAGAGACC[T/C] A CCAGAGGTTT AACCTCTGGGCCAGGA AAGAGGATGAGTGACA GGTGGCTTAGAGGCCC C AAGCAGTCGA 18 MARC0046857 43251114 GG [A/G] GAGTAAAGGG ATTATCTCTATGTACATAT CTTTGAAGGA CAAAAAAATCAGAGAA GAAGATTACT AAATGAACAATCTAGGT GTTCTCTCTTT TATCATTA[A/G] AAACAT GAAATGTTT TTCAAAGAGAGAACAG TAATCTTCTCCTTCAAA GCCCTTTACTC ACAATT CTTC 18 MARC0077194 43266873 AA [A/G] AACTCAGGAA AGTGACCTGG AACTCA AGTGTAACCC GGAAAGTGTAACCCAG AGGTGTCCTC GTGTCCTCAAAGAGAA AAAGAGAAG GGGGGGAGATTG [A/G] GGGGGAGATT TTATATAAAAATAACAAA G AATAAAAACAAAAAATA AACAAAAAACACAGGA AACTGCTTG 18 ALGA0108769 43277580 CC [T/C] GAGGCATGCT AGACACGGAG GAGGCA GTAGTATCTTT TGCTGTAGTATCTTTCA CAGCCATTAC GCCATTACCTGGGAGC CTGGGAGCAG AGGTTACTGAG [T/C]CT GTTACTGAG TGGTTCAGTAAAGNNNN NNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNN NNNNNNNNN 18 H3GA0050799 43325649 CC [T/C] GCAGGATATA CTGGAAAATG GCAGGAT AACAGATATAT ATAAACAGATATATAGA AGATGTAATC TGTAATCAAGCTTCCT AAGCTTCCTC CCTCCACCAA [T/C]GAA CTCCACCAA AACAGATCTTCTGCATG GCCGAACTCCACCTTGC CTCCTCAGAGGCCCTTT CACTTC 18 ALGA0098120 43337188 CC [T/C] CCTGGAAGTG TGCCATTTTTATCAAAGT GGCCAGACTG TCAACCTTTCATCATCAT TAGATAAAGC GTAAGTAGTCAAAGCTG GTTTGATGTG TTACAAG[T/C] CTACTGC TCAGCAGTAG TGACACATCAAACGCT TTATCTACAGTCTGGC CCACTTCCAGG GAGAT GAAAA 18 ALGA0098123 43370488 GG [A/G] CCCAACACAG AGGTTAAGAA CCCAAC TGTCATGAGG ACAGTGTCATGAGGAT ATGTGGGTTC GTGGGTTCAATCCCTG AATCCCTGCC CCCTCACTCAGT [A/G]G CTCACTCAGT GTTGKGGATCTGGTGTT GCCGCAAGCTGGCAGTA CAGGTTGTAGATGTGGC TCAGATCC 18 ALGA0098128 43394468 GG [A/G] CTTTGTTTTAG CATGATCAATGACAGAT GAGGGAGTCC GCCAGTCCTGAATTACC TCAGCCAAGT AAGGAGAATACCCCAGG CTAGCTAGAT GCTGCCCTT[A/G] ACTAT GGGGATAGT CCCCATCTAGCTAGAC TTGGCTGAGGACTCCC TCCTAAAACAAAG AATA ATGCAT CCTCTAGAAA CCTTAGTTCTCTTCCACA ASGA0079719 43493743 CC [T/C] CCTTGAAGGC TGGCCTCTCATTCTCCAC 18 TCTTGCATTCT TAGACTCCACTGGGCTT TTCTTGGAGC CTTTACA[T/C] GGAAGC CCAGCTTCC TGGGCTCCAAGAAAGA ATGCAAGAGCCTTCAA GGTTTCTAGAGG CCCA GGCACA 18 ASGA0079728 43556022 GG [A/G] GTCTTATCAAA GGTCGAACTCAAGCAGA TGTGACCTGC AGGTCCAAGATGTGAAC TTCTGGCCAT ACCAAGCTGTCCTACCC GCAGGGTGGG CCAGGGCTG[A/G] CACA AAGAATGTG TTCTTCCCACCCTGCA TGGCCAGAAGCAGGTC ACATTTGATAAGAC TTT TCTTTAA 20th 18 ASGA0080429 58958866 CC [T/C] GATTTAGGAA GGAAAGGAAG GATTTA segment AGCACTGAGT GGAAAGCACTGAGTAT ATCAGCCATC CAGCCATCCAGCCAAG CAGCCAAGGT GTAGGTACAGGA [T/C]G AGGTACAGGA GCATTCAAGCACAGAGT CCCCAGGAAGAGCTGCA GTCCTGATGCCCCATGCT GACACTG 18 MARC0052755 59086075 GG [A/G] TTCTCATACAC GAGAGCCAAT TTCTCAT CATCCAATAA ACACCATCCAATAAAC ACACTGACCA ACTGACCACATCCTGA CATCCTGACC CCAGAGCTAAG [A/G]AC AGAGCTAAG TGTATAATGCTAAGCATA TCTAGG 18 ALGA0098918 59451170 GG [A/G] TCTGCATGTAA GGAGTCTGAT TCTGCAT TTTAGTGCAG GTAATTTAGTGCAGAG AGTGCCAGAA TGCCAGAATCCCGAGT TCCCGAGTAG AGCTGGGTTCC [A/G]GT CTGGGTTCC CCTGGCTTGCCATTTACT AGGTACGTAACCCTGGG CACATTGCTGACCTGAT CCGTGC 18 ASGA0080432 59520434 GG [A/G] GGAAGCCATT TGATGACACA GGAAGC TTCCTCCTCCC CATTTTCCTCCTCCCGT GTGGACACAA GGACACAATGAATGTG TGAATGTGCA CAGCTACACAC [A/G]RA GCTACACAC GCAGTTCTCGCCGACAG AAATCTGGACGCCGAGA AAAGAGAGACATTCAGT TGAATGA 18 ALGA0098922 59553266 CC [T/C] AAATTTTAAG GAGAAAAAAA AAATTT TTAAAACAAG TAAGTTAAAACAAGCT CTGTGCCCAG GTGCCCAGAGGAACAT AGGAACATAT ATAGTCTGGCTC [T/C]T AGTCTGGCTC CAATGTCACAAGAAAAA TATGGGAAAGCTGAGCT TAGAGCTAAAAAGAACT ACTGATAT 18 INRA0056206 59600842 GG [A/G] AACCGAAATT AGCCAGATGC AACCGA GTGTTTTCATC AATTGTGTTTTCATCCC CCTGAGTTTC TGAGTTTCCAACTGTT CAACTGTTCTA CTATTTAAGCA [A/G]AA TTTAAGCA TTTTCTCTATTAAAATTT GACTTTAAAATTTTAGA GACACATTTTTATCTTTT ACATT 18 INRA0056207 59625294 CC [T/C] TAATCCTGAA ATCTATGTATCTTGGACC AATAGCCGGG AAAATGGTTTTAGTTACT AAAATTCTTG AAAATTCTGTTTTAACAT AAAGAGAAG ACAATA[T/C] CCCAAAC GACAGTTTGG TGTCCTTCTCTTTCAA G GAATTTTCCCGGCTATT TTCAGGATTA TCCCTCC AGA 18 ASGA0080435 59655603 CC [T/C] GATATATTTAC ACAAAGAGTG GATATAT ATGTGGGACT TTACATGTGGGACTGA GAGTCACTCG GTCACTCGGCTGCACA GCTGCACACC CCTGAAGCTAA [T/C]GC TGAAGCTAA AACATTATATATACATCA ACTATACTCAAATAAAAC TAATTTAAAAAAGAAAA TTAAA 18 MARC0068495 59713388 GG [A/G] AAATCTAGAG CTGTAACAGTTCAGGCA GTGGGAAACT ACTGTCTCCCAGGGTTT TTGAGACAGC TGCTAAGACACTGGGCG CTATTCCATTA AACCTACAG[A/G] TCCC GACTTGGGA AAGTCTAATGGAATAG GCTGTCTCAAAGTTTC CCACCTCTAGATTT ATA CAGGCAC 18 ASGA0080436 59739721 CC [T/C] TTAATCAACTT GCACATGACTAAAATGC CAGACATTTC CGAGGGAGGGAAGCGA CTCTTTGCCTC ACTGAGCCGAGCTATCA CTGCTTTGAC AAGTGCTGAA[T/C] GCC GAAAAGGC TTTTCGTCAAAGCAGG AGGCAAAGAGGAAATG TCTGAAGTTGATTAA TG AGATACGA 18 MARC0003370 59753551 CC [T/C] CCTTCCATTG CCCTGCACTA CCTTCCA GCTCTAGAAT TTGGCTCTAGAATAAG AAGCTCCGGA CTCCGGATTGTACAAA TTGTACAAAT TCTGACTCGGT [T/C]GT CTGACTCGGT ACCCTGTTTAAAATCCTT CAGTAGCTT 18 ASGA0085659 59827202 CC [T/C] AGTTGGTTTC CATGGATTCC AGTTGGT GTTGCTGCTG TTCGTTGCTGCTGAGC AGCCACAACA CACAACAGGAATTCAT GGAATTCATAT ATTAAGAACAT [T/C]TTT TAAGAACAT AAGYGATACTAGNNNNN NNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNN NNNNNNNN

In table 1, N represents A, T, C, or G. In table 1, underlines in source sequence label the regions to which probes correspond (containing two cases, the nucleotide sequence of probe is same as that of underline region, the nucleotide sequence of probe is reversely complementary to that of underline region). In table 1, when source type of SNP is [T/G], heterozygous type is TG; when source type of SNP is [A/G], heterozygous type is AG; when source type of SNP is [T/C], heterozygous type is TC; and when source type of SNP is [A/C], heterozygous type is AC.

Example 2. Identifying Wuzhishan Miniature Pig Inbred Line and Non-Wuzhishan Miniature Pig Inbred Line by Using 145 SNPs

1. Selecting experimental animals, collecting blood from ear veins, and extracting genomic DNA from blood samples;

2. Hybridizing the genomic DNA obtained in Step 1 with nucleic acid chips immobilized with 145 probes (these 145 probes are single-stranded DNA molecules shown as Sequences 1-145 in the sequence listing, respectively);

3. After accomplishing Step 2, terminal extensions were performed on each point in nucleic acid chips, so as to obtain the genotypes of 145 SNP sites in genomic DNA;

4. Whole genome sequencing was performed on genomic DNA obtained in Step 1 to obtain the genotypes of 145 SNP sites in genomic DNA.

The results demonstrated that the results of Step 3 are completely consistent with those of Step 4.

Genotypes of 145 SNP sites of 48 experimental animals of Wuzhishan miniature pig inbred line are all homozygous type (genotypes described in Table 1 of Example 1). Genotypes of 145 SNP sites of 16 experimental animals of Hainan Wuzhishan pig are all heterozygous type.

INDUSTRIAL APPLICATION

The present invention is of a significant value for the identification of germplasm resource of Wuzhishan miniature pig inbred line. The method of the present invention can be used for breeding of Wuzhishan miniature pig inbred line, preliminarily screening all the pigs in the pig population to be tested, weeding out non-Wuzhishan miniature pig inbred line, finding out candidate Wuzhishan miniature pig inbred line, and further confirming in combination with other methods. The present invention also can be applied to test whether the purchased Wuzhishan miniature pig inbred line is counterfeit. 

1. A method for auxiliarily identifying whether a pig to be tested is Wuzhishan miniature pig inbred line, comprising the following steps: Testing genotype based on 145 SNP sites of the pig to be tested; If all the standards of the following (1) to (145) are satisfied, the pig to be tested is a candidate for Wuzhishan miniature pig inbred line: (1) M1GA0025062 the genotype of SNP site is GG; (2) DRGA0017627 the genotype of SNP site is GG; (3) H3GA0056137 the genotype of SNP site is CC; (4) MARC0039661 the genotype of SNP site is AA; (5) ASGA0085025 the genotype of SNP site is TT; (6) ASGA0098570 the genotype of SNP site is GG; (7) MARC0063358 the genotype of SNP site is AA; (8) ASGA0085437 the genotype of SNP site is GG; (9) MARC0096709 the genotype of SNP site is CC; (10) H3GA0052387 the genotype of SNP site is GG; (11) ASGA0000264 the genotype of SNP site is TT; (12) ALGA0000195 the genotype of SNP site is TT; (13) H3GA0001444 the genotype of SNP site is AA; (14) ASGA0002293 the genotype of SNP site is CC; (15) INRA0001752 the genotype of SNP site is GG; (16) H3GA0001445 the genotype of SNP site is GG; (17) ALGA0002595 the genotype of SNP site is TT; (18) ALGA0002600 the genotype of SNP site is GG; (19) DRGA0000563 the genotype of SNP site is CC; (20) DRGA0000565 the genotype of SNP site is CC; (21) INRA0001761 the genotype of SNP site is AA; (22) MARC0104045 the genotype of SNP site is CC; (23) ALGA0002601 the genotype of SNP site is CC; (24) DRGA0000569 the genotype of SNP site is AA; (25) ALGA0002608 the genotype of SNP site is TT; (26) DRGA0000568 the genotype of SNP site is CC; (27) ALGA0002604 the genotype of SNP site is GG; (28) MARC0048118 the genotype of SNP site is TT; (29) ASGA0004229 the genotype of SNP site is CC; (30) MARC0007088 the genotype of SNP site is CC; (31) MARC0000061 the genotype of SNP site is GG; (32) MARC0094747 the genotype of SNP site is AA; (33) MARC0007969 the genotype of SNP site is AA; (34) ASGA0106092 the genotype of SNP site is CC; (35) ALGA0119806 the genotype of SNP site is GG; (36) INRA0003745 the genotype of SNP site is AA; (37) INRA0003746 the genotype of SNP site is TT; (38) ALGA0005490 the genotype of SNP site is CC; (39) H3GA0002538 the genotype of SNP site is CC; (40) INRA0003749 the genotype of SNP site is CC; (41) H3GA0002539 the genotype of SNP site is GG; (42) M1GA0001099 the genotype of SNP site is CC; (43) MARC0111831 the genotype of SNP site is AA; (44) ASGA0005566 the genotype of SNP site is CC; (45) ALGA0007578 the genotype of SNP site is CC; (46) ASGA0005568 the genotype of SNP site is CC; (47) INRA0005765 the genotype of SNP site is AA; (48) MARC0095347 the genotype of SNP site is CC; (49) MARC0039015 the genotype of SNP site is CC; (50) DRGA0001892 the genotype of SNP site is GG; (51) ALGA0007580 the genotype of SNP site is GG; (52) ALGA0007583 the genotype of SNP site is TT; (53) MARC0095915 the genotype of SNP site is CC; (54) ASGA0005571 the genotype of SNP site is AA; (55) MARC0014134 the genotype of SNP site is AA; (56) MARC0024233 the genotype of SNP site is AA; (57) H3GA0003548 the genotype of SNP site is GG; (58) ALGA0007591 the genotype of SNP site is CC; (59) MARC0000195 the genotype of SNP site is CC; (60) ALGA0038697 the genotype of SNP site is CC; (61) H3GA0020000 the genotype of SNP site is CC; (62) ALGA0038703 the genotype of SNP site is CC; (63) H3GA0020002 the genotype of SNP site is GG; (64) DRGA0007138 the genotype of SNP site is CC; (65) ASGA0031284 the genotype of SNP site is CC; (66) ASGA0031285 the genotype of SNP site is AA; (67) MARC0025042 the genotype of SNP site is GG; (68) H3GA0020006 the genotype of SNP site is TT; (69) ALGA0038729 the genotype of SNP site is CC; (70) ALGA0038731 the genotype of SNP site is GG; (71) ASGA0031321 the genotype of SNP site is GG; (72) ASGA0031322 the genotype of SNP site is GG; (73) ALGA0038747 the genotype of SNP site is AA; (74) ASGA0033095 the genotype of SNP site is TT; (75) ALGA0040854 the genotype of SNP site is GG; (76) ALGA0040856 the genotype of SNP site is AA; (77) ALGA0040857 the genotype of SNP site is GG; (78) ASGA0033096 the genotype of SNP site is GG; (79) INRA0025180 the genotype of SNP site is AA; (80) ALGA0040859 the genotype of SNP site is CC; (81) H3GA0021216 the genotype of SNP site is CC; (82) ASGA0033098 the genotype of SNP site is CC; (83) H3GA0021221 the genotype of SNP site is CC; (84) MARC0014540 the genotype of SNP site is CC; (85) ASGA0033103 the genotype of SNP site is CC; (86) DIAS0000557 the genotype of SNP site is AA; (87) ASGA0033116 the genotype of SNP site is AA; (88) ASGA0036835 the genotype of SNP site is CC; (89) MARC0031932 the genotype of SNP site is CC; (90) ASGA0036838 the genotype of SNP site is CC; (91) H3GA0023523 the genotype of SNP site is GG; (92) ALGA0045460 the genotype of SNP site is TT; (93) MARC0005927 the genotype of SNP site is GG; (94) MARC0005928 the genotype of SNP site is AA; (95) ASGA0036842 the genotype of SNP site is CC; (96) ASGA0036846 the genotype of SNP site is CC; (97) MARC0098637 the genotype of SNP site is TT; (98) M1GA0011035 the genotype of SNP site is TT; (99) DRGA0008230 the genotype of SNP site is GG; (100) ASGA0036855 the genotype of SNP site is CC; (101) ALGA0097277 the genotype of SNP site is GG; (102) MARC0080197 the genotype of SNP site is CC; (103) H3GA0050489 the genotype of SNP site is TT; (104) ALGA0097281 the genotype of SNP site is TT; (105) H3GA0050490 the genotype of SNP site is CC; (106) ALGA0097282 the genotype of SNP site is TT; (107) ASGA0079089 the genotype of SNP site is CC; (108) INRA0055354 the genotype of SNP site is GG; (109) ASGA0079091 the genotype of SNP site is TT; (110) ASGA0079090 the genotype of SNP site is CC; (111) H3GA0050491 the genotype of SNP site is AA; (112) MARC0056017 the genotype of SNP site is TT; (113) MARC0055759 the genotype of SNP site is AA; (114) H3GA0050495 the genotype of SNP site is GG; (115) ALGA0097291 the genotype of SNP site is GG; (116) ALGA0097290 the genotype of SNP site is GG; (117) CASI0006683 the genotype of SNP site is GG; (118) ASGA0079098 the genotype of SNP site is CC; (119) ALGA0097297 the genotype of SNP site is GG; (120) H3GA0054426 the genotype of SNP site is TT; (121) ALGA0098112 the genotype of SNP site is GG; (122) MARC0089391 the genotype of SNP site is GG; (123) ASGA0089892 the genotype of SNP site is AA; (124) ASGA0097792 the genotype of SNP site is CC; (125) MARC0046857 the genotype of SNP site is GG; (126) MARC0077194 the genotype of SNP site is AA; (127) ALGA0108769 the genotype of SNP site is CC; (128) H3GA0050799 the genotype of SNP site is CC; (129) ALGA0098120 the genotype of SNP site is CC; (130) ALGA0098123 the genotype of SNP site is GG; (131) ALGA0098128 the genotype of SNP site is GG; (132) ASGA0079719 the genotype of SNP site is CC; (133) ASGA0079728 the genotype of SNP site is GG; (134) ASGA0080429 the genotype of SNP site is CC; (135) MARC0052755 the genotype of SNP site is GG; (136) ALGA0098918 the genotype of SNP site is GG; (137) ASGA0080432 the genotype of SNP site is GG; (138) ALGA0098922 the genotype of SNP site is CC; (139) INRA0056206 the genotype of SNP site is GG; (140) INRA0056207 the genotype of SNP site is CC; (141) ASGA0080435 the genotype of SNP site is CC; (142) MARC0068495 the genotype of SNP site is GG; (143) ASGA0080436 the genotype of SNP site is CC; (144) MARC0003370 the genotype of SNP site is CC; (145) ASGA0085659 the genotype of SNP site is CC; If any standard of the above (1) to (145) is not satisfied, the pig to be tested is a candidate for non-Wuzhishan miniature pig inbred line.
 2. The method of claim 1, wherein the pig to be tested is Wuzhishan miniature pig inbred line or Hainan Wuzhishan pig.
 3. The method of claim 1, wherein the Wuzhishan miniature pig inbred line belongs to any one of F₂₀ to F₂₂ generations.
 4. The method of claim 1, wherein the Wuzhishan miniature pig inbred line belongs to F₂₀ generation or generations later than F₂₀ generation.
 5. The method of claim 1, wherein in the method, a specific chip is employed to test genotypes based on the 145 SNP sites of the pig to be tested; the specific chip is a nucleic acid chip in which single-stranded DNA molecules shown as Sequences 1-145 in the sequence listing are immobilized at different points, respectively.
 6. A nucleic acid chip, in which single-stranded DNA molecules shown as Sequences 1-145 in the sequence listing are immobilized at different points, respectively.
 7. A method, comprising the following (a) or (b): (a) auxiliarily identifying with the nucleic acid chip of claim 6 whether a pig to be tested is Wuzhishan miniature pig inbred line; or (b) auxiliarily identifying with the nucleic acid chip of claim 6 whether a pig population to be tested is Wuzhishan miniature pig inbred line population.
 8. A method for auxiliarily identifying whether a pig population to be tested is Wuzhishan miniature pig inbred line population, comprising the following steps: Randomly sampling from the pig population to be tested to obtain samples to be tested, then testing the genotypes based on 145 SNP sites of each of samples to be tested, and if all the samples to be tested satisfy all the standards of the following (1) to (145), the pig population to be tested is a candidate for Wuzhishan miniature pig inbred line population: (1) M1GA0025062 the genotype of SNP site is GG; (2) DRGA0017627 the genotype of SNP site is GG; (3) H3GA0056137 the genotype of SNP site is CC; (4) MARC0039661 the genotype of SNP site is AA; (5) ASGA0085025 the genotype of SNP site is TT; (6) ASGA0098570 the genotype of SNP site is GG; (7) MARC0063358 the genotype of SNP site is AA; (8) ASGA0085437 the genotype of SNP site is GG; (9) MARC0096709 the genotype of SNP site is CC; (10) H3GA0052387 the genotype of SNP site is GG; (11) ASGA0000264 the genotype of SNP site is TT; (12) ALGA0000195 the genotype of SNP site is TT; (13) H3GA0001444 the genotype of SNP site is AA; (14) ASGA0002293 the genotype of SNP site is CC; (15) INRA0001752 the genotype of SNP site is GG; (16) H3GA0001445 the genotype of SNP site is GG; (17) ALGA0002595 the genotype of SNP site is TT; (18) ALGA0002600 the genotype of SNP site is GG; (19) DRGA0000563 the genotype of SNP site is CC; (20) DRGA0000565 the genotype of SNP site is CC; (21) INRA0001761 the genotype of SNP site is AA; (22) MARC0104045 the genotype of SNP site is CC; (23) ALGA0002601 the genotype of SNP site is CC; (24) DRGA0000569 the genotype of SNP site is AA; (25) ALGA0002608 the genotype of SNP site is TT; (26) DRGA0000568 the genotype of SNP site is CC; (27) ALGA0002604 the genotype of SNP site is GG; (28) MARC0048118 the genotype of SNP site is TT; (29) ASGA0004229 the genotype of SNP site is CC; (30) MARC0007088 the genotype of SNP site is CC; (31) MARC0000061 the genotype of SNP site is GG; (32) MARC0094747 the genotype of SNP site is AA; (33) MARC0007969 the genotype of SNP site is AA; (34) ASGA0106092 the genotype of SNP site is CC; (35) ALGA0119806 the genotype of SNP site is GG; (36) INRA0003745 the genotype of SNP site is AA; (37) INRA0003746 the genotype of SNP site is TT; (38) ALGA0005490 the genotype of SNP site is CC; (39) H3GA0002538 the genotype of SNP site is CC; (40) INRA0003749 the genotype of SNP site is CC; (41) H3GA0002539 the genotype of SNP site is GG; (42) M1GA0001099 the genotype of SNP site is CC; (43) MARC0111831 the genotype of SNP site is AA; (44) ASGA0005566 the genotype of SNP site is CC; (45) ALGA0007578 the genotype of SNP site is CC; (46) ASGA0005568 the genotype of SNP site is CC; (47) INRA0005765 the genotype of SNP site is AA; (48) MARC0095347 the genotype of SNP site is CC; (49) MARC0039015 the genotype of SNP site is CC; (50) DRGA0001892 the genotype of SNP site is GG; (51) ALGA0007580 the genotype of SNP site is GG; (52) ALGA0007583 the genotype of SNP site is TT; (53) MARC0095915 the genotype of SNP site is CC; (54) ASGA0005571 the genotype of SNP site is AA; (55) MARC0014134 the genotype of SNP site is AA; (56) MARC0024233 the genotype of SNP site is AA; (57) H3GA0003548 the genotype of SNP site is GG; (58) ALGA0007591 the genotype of SNP site is CC; (59) MARC0000195 the genotype of SNP site is CC; (60) ALGA0038697 the genotype of SNP site is CC; (61) H3GA0020000 the genotype of SNP site is CC; (62) ALGA0038703 the genotype of SNP site is CC; (63) H3GA0020002 the genotype of SNP site is GG; (64) DRGA0007138 the genotype of SNP site is CC; (65) ASGA0031284 the genotype of SNP site is CC; (66) ASGA0031285 the genotype of SNP site is AA; (67) MARC0025042 the genotype of SNP site is GG; (68) H3GA0020006 the genotype of SNP site is TT; (69) ALGA0038729 the genotype of SNP site is CC; (70) ALGA0038731 the genotype of SNP site is GG; (71) ASGA0031321 the genotype of SNP site is GG; (72) ASGA0031322 the genotype of SNP site is GG; (73) ALGA0038747 the genotype of SNP site is AA; (74) ASGA0033095 the genotype of SNP site is TT; (75) ALGA0040854 the genotype of SNP site is GG; (76) ALGA0040856 the genotype of SNP site is AA; (77) ALGA0040857 the genotype of SNP site is GG; (78) ASGA0033096 the genotype of SNP site is GG; (79) INRA0025180 the genotype of SNP site is AA; (80) ALGA0040859 the genotype of SNP site is CC; (81) H3GA0021216 the genotype of SNP site is CC; (82) ASGA0033098 the genotype of SNP site is CC; (83) H3GA0021221 the genotype of SNP site is CC; (84) MARC0014540 the genotype of SNP site is CC; (85) ASGA0033103 the genotype of SNP site is CC; (86) DIAS0000557 the genotype of SNP site is AA; (87) ASGA0033116 the genotype of SNP site is AA; (88) ASGA0036835 the genotype of SNP site is CC; (89) MARC0031932 the genotype of SNP site is CC; (90) ASGA0036838 the genotype of SNP site is CC; (91) H3GA0023523 the genotype of SNP site is GG; (92) ALGA0045460 the genotype of SNP site is TT; (93) MARC0005927 the genotype of SNP site is GG; (94) MARC0005928 the genotype of SNP site is AA; (95) ASGA0036842 the genotype of SNP site is CC; (96) ASGA0036846 the genotype of SNP site is CC; (97) MARC0098637 the genotype of SNP site is TT; (98) M1GA0011035 the genotype of SNP site is TT; (99) DRGA0008230 the genotype of SNP site is GG; (100) ASGA0036855 the genotype of SNP site is CC; (101) ALGA0097277 the genotype of SNP site is GG; (102) MARC0080197 the genotype of SNP site is CC; (103) H3GA0050489 the genotype of SNP site is TT; (104) ALGA0097281 the genotype of SNP site is TT; (105) H3GA0050490 the genotype of SNP site is CC; (106) ALGA0097282 the genotype of SNP site is TT; (107) ASGA0079089 the genotype of SNP site is CC; (108) INRA0055354 the genotype of SNP site is GG; (109) ASGA0079091 the genotype of SNP site is TT; (110) ASGA0079090 the genotype of SNP site is CC; (111) H3GA0050491 the genotype of SNP site is AA; (112) MARC0056017 the genotype of SNP site is TT; (113) MARC0055759 the genotype of SNP site is AA; (114) H3GA0050495 the genotype of SNP site is GG; (115) ALGA0097291 the genotype of SNP site is GG; (116) ALGA0097290 the genotype of SNP site is GG; (117) CASI0006683 the genotype of SNP site is GG; (118) ASGA0079098 the genotype of SNP site is CC; (119) ALGA0097297 the genotype of SNP site is GG; (120) H3GA0054426 the genotype of SNP site is TT; (121) ALGA0098112 the genotype of SNP site is GG; (122) MARC0089391 the genotype of SNP site is GG; (123) ASGA0089892 the genotype of SNP site is AA; (124) ASGA0097792 the genotype of SNP site is CC; (125) MARC0046857 the genotype of SNP site is GG; (126) MARC0077194 the genotype of SNP site is AA; (127) ALGA0108769 the genotype of SNP site is CC; (128) H3GA0050799 the genotype of SNP site is CC; (129) ALGA0098120 the genotype of SNP site is CC; (130) ALGA0098123 the genotype of SNP site is GG; (131) ALGA0098128 the genotype of SNP site is GG; (132) ASGA0079719 the genotype of SNP site is CC; (133) ASGA0079728 the genotype of SNP site is GG; (134) ASGA0080429 the genotype of SNP site is CC; (135) MARC0052755 the genotype of SNP site is GG; (136) ALGA0098918 the genotype of SNP site is GG; (137) ASGA0080432 the genotype of SNP site is GG; (138) ALGA0098922 the genotype of SNP site is CC; (139) INRA0056206 the genotype of SNP site is GG; (140) INRA0056207 the genotype of SNP site is CC; (141) ASGA0080435 the genotype of SNP site is CC; (142) MARC0068495 the genotype of SNP site is GG; (143) ASGA0080436 the genotype of SNP site is CC; (144) MARC0003370 the genotype of SNP site is CC; (145) ASGA0085659 the genotype of SNP site is CC; If more than one of the samples to be tested do not satisfy all the standards of the above (1) to (145), the pig population to be tested is a candidate for non-Wuzhishan miniature pig inbred line population.
 9. The method of claim 8, wherein the pig to be tested is Wuzhishan miniature pig inbred line or Hainan Wuzhishan pig.
 10. The method of claim 8, wherein the Wuzhishan miniature pig inbred line belongs to any one of F₂₀ to F₂₂ generations.
 11. The method of claim 8, wherein the Wuzhishan miniature pig inbred line belongs to F₂₀ generation or generations later than F₂₀ generation.
 12. The method of claim 8, wherein in the method, a specific chip is employed to test genotypes based on the 145 SNP sites of each of the samples to be tested; the specific chip is nucleic acid chip in which single-stranded DNA molecules shown as Sequences 1-145 in the sequence listing are immobilized at different points, respectively. 